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Product Info Summary
| SKU: | PA1774 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-SNAP23 Antibody Picoband®
SKU/Catalog Number
PA1774
BA2805 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNAP23 Antibody catalog # PA1774. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAP23 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1774)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1774 is reactive to SNAP23 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.4 kDa
Background of SNAP23
SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1774 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human HEL whole cell, human CACO-2 whole cell, human THP-1 whole cell, human U2OS whole cell, human 293T whole cell
IHC: Human Placenta Tissue, human tonsil tissue
IHC-F: Human Placenta Tissue
ICC/IF: T47D cell
ICC: K562 Cell
FCM: HEPG2 cell
Validation Images & Assay Conditions
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Western blot analysis of SNAP23 using anti-SNAP23 antibody (PA1774).
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (PA1774) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 23 kDa. The expected band size for SNAP23 is at 23 kDa.
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Anti-SNAP23 antibody, PA1774, IHC(P)
IHC(P): Human Placenta Tissue
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Anti-SNAP23 antibody, PA1774, IHC(F)
IHC(F): Human Placenta Tissue
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Anti-SNAP23 antibody, PA1774,ICC
ICC: K562 Cell
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IF analysis of SNAP23 using anti-SNAP23 antibody (PA1774).
SNAP23 was detected in immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SNAP23 Antibody (PA1774) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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IHC analysis of SNAP23 using anti-SNAP23 antibody (PA1774).
SNAP23 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SNAP23 Antibody (PA1774) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Flow Cytometry analysis of HEPG2 cells using anti-SNAP23 antibody (PA1774).
Overlay histogram showing HEPG2 cells stained with PA1774 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP23 Antibody (PA1774, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SNAP23 Antibody Picoband® (PA1774)
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7 Customer Q&As for Anti-SNAP23 Antibody Picoband®
Question
Can you help my question with product PA1774, anti-SNAP23 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1774 anti-SNAP23 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-11
Question
I was wanting to use your anti-SNAP23 antibody for ICC for human placenta testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta testis identification?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
As indicated on the product datasheet, PA1774 anti-SNAP23 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-31
Question
I see that the anti-SNAP23 antibody PA1774 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-24
Question
We are currently using anti-SNAP23 antibody PA1774 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-SNAP23 antibody (PA1774) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-SNAP23 antibody PA1774. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-02
Question
Is a blocking peptide available for product anti-SNAP23 antibody (PA1774)?
G. Mitchell
Verified customer
Asked: 2017-04-19
Answer
We do provide the blocking peptide for product anti-SNAP23 antibody (PA1774). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-04-19
Question
Is there a BSA free version of anti-SNAP23 antibody PA1774 available?
P. Collins
Verified customer
Asked: 2015-03-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP23 antibody PA1774 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-03-31
