Anti-SNAP23 Antibody

SNAP23 antibody

Boster Bio Anti-SNAP23 Antibody catalog # PA1774. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1774
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-SNAP23 Antibody

View all SNAP23 Antibodies

SKU/Catalog Number

PA1774

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNAP23 Antibody catalog # PA1774. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNAP23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1774)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1774 is reactive to SNAP23 in Human, Mouse, Rat

Applications

PA1774 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

105 kDa

Calculated molecular weight

23.354kDa

Background of SNAP23

SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For SNAP23 (Source: Uniprot.org, NCBI)

Gene Name

SNAP23

Full Name

Synaptosomal-associated protein 23

Weight

23.354kDa

Superfamily

SNAP-25 family

Alternative Names

HsT17016; SNAP23; SNAP-23; SNAP23A; SNAP23B; synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; Vesicle-membrane fusion protein SNAP-23 SNAP23 HsT17016, SNAP-23A, SNAP23B, SNAP23 synaptosome associated protein 23 synaptosomal-associated protein 23|synaptosomal-associated protein, 23kD|synaptosomal-associated protein, 23kDa|synaptosome associated protein 23kDa|vesicle-membrane fusion protein SNAP-23

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SNAP23, check out the SNAP23 Infographic

SNAP23 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SNAP23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1774 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang,Y.,Pang,J.,Wang,Q.,Yan,L.,Wang,L.,Xing,Z.,Wang,C.,Zhang,J., Dong,L.,Delivering Antisense Oligonucleotides across the Blood‐Brain Barrier by Tumor Cell‐Derived Small Apoptotic Bodies.Adv.Sci.2021,2004929.https://doi.org/10.1002/advs.202004929
Species: Mouse
PA1774 usage in article: APP:TRANSCYTOSIS ANALYSIS, SAMPLE:B16F10 CELL, DILUTION:NA

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7 Customer Q&As for Anti-SNAP23 Antibody

Question

Can you help my question with product PA1774, anti-SNAP23 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1774 anti-SNAP23 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-11

Question

I was wanting to use your anti-SNAP23 antibody for ICC for human placenta testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta testis identification?

Verified Customer

Verified customer

Asked: 2018-12-31

Answer

As indicated on the product datasheet, PA1774 anti-SNAP23 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-31

Question

I see that the anti-SNAP23 antibody PA1774 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-24

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-24

Question

We are currently using anti-SNAP23 antibody PA1774 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

The anti-SNAP23 antibody (PA1774) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-13

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-SNAP23 antibody PA1774. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-02

Question

Is a blocking peptide available for product anti-SNAP23 antibody (PA1774)?

G. Mitchell

Verified customer

Asked: 2017-04-19

Answer

We do provide the blocking peptide for product anti-SNAP23 antibody (PA1774). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-04-19

Question

Is there a BSA free version of anti-SNAP23 antibody PA1774 available?

P. Collins

Verified customer

Asked: 2015-03-31

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP23 antibody PA1774 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-03-31

Order DetailsPrice
PA1774

100μg

$370
PA1774-10ug

10μg sample (liquid)

$99
PA1774-Biotin

100 μg Biotin conjugated

$570
PA1774-Cy3

100 μg Cy3 conjugated

$570
PA1774-Dylight488

100 μg Dylight488 conjugated

$570
PA1774-Dylight550

100 μg Dylight550 conjugated

$570
PA1774-Dylight594

100 μg Dylight594 conjugated

$570
PA1774-FITC

100 μg FITC conjugated

$570
PA1774-HRP

100 μg HRP conjugated

$570
PA1774-APC

100 μg APC conjugated

$670
PA1774-PE

100 μg PE conjugated

$670
PA1774-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1774
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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