Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 23(SNAP23) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Anti-SNAP23 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
Kruppel-Like Factor 6 (KLF6) is a nuclear protein which has 3 zinc fingers. The zinc fingers of KLF6 are accountable for the specific DNA binding with the guanine-rich core promoter factors. The central region is involved in activation or posttranslational regulatory pathways, and the acidic N terminal domain takes part in the process of transcriptional activation.
|Product Name||Anti-SNAP23 Antibody
See all SNAP23 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for SNAP 23/SNAP23 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. SNAP 23/SNAP23 information: Molecular Weight: 23354 MW; Subcellular Localization: Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane; Tissue Specificity: Ubiquitous. Highest levels where found in placenta.|
|Cite This Product||Anti-SNAP23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1774)|
|Similar Products From
|Anti-SNAP23 Antibody may replace the following items: sc 166244|sc 22643|sc 22644|sc 373743|sc 374060|sc 374215|sc 50371.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23(192-211aa DTNRDRIDIANARAKKLIDS), different from the related rat and mouse sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SNAP23 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8773|
|Species Of This Entry||Human|
|Protein Name||Synaptosomal-associated protein 23|
|Alternative Names||SNAP23|HsT17016; SNAP23; SNAP-23; SNAP23A; SNAP23B; synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; Vesicle-membrane fusion protein SNAP-23|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Palmitoylation; Biotinylation; Dephosphorylation; Acylation; Glycosylation; Oxidation; Ribosylation; Myristoylation|
|Subcellular Localization||Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.|
|Gene Location||15q15.1-q15.2, on Chromasome 15, gene sequence: NC_000015.10 (42491244..42533061)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SNAP23 (Source: Uniprot.org, NCBI)
|Protein Function||Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.|
|Tissue Specificity||Ubiquitous. Highest levels where found in placenta.|
|Interacting Proteins||NAPA; NAPB; STX11; VAMP3; ZDHHC17|
|Research Areas||Membrane Trafficking and Chaperones; Neuronal Cell Markers; Neuroscience; Neurotransmission|
|Related Diseases||Tetanus; Diabetes Mellitus, Non-insulin-dependent; Insulin Resistance; Diabetes Mellitus; Inflammation; Tissue Adhesions; Pancreatitis; Nervousness; Leukemia; Glioma; Fibrosis; Pancreatitis, Alcoholic; Cystic Fibrosis; Thrombus; Allergy; Obesity; Disease Of Adrenal Medulla; Neoplasms; Basophilic Leukemia; Anaphylaxis|
|Related Pathways||Exocytosis; Secretion; Membrane Fusion; Transport; Localization; Vesicle Fusion; Snare Complex Assembly; Secretory Pathway; Glucose Transport; Vesicle Docking; Cell Migration; Synaptic Vesicle Exocytosis; Rna Interference; Platelet Activation; Immune Response; Proteolysis; Membrane Docking; Endocytosis; Mast Cell Degranulation; Synaptic Transmission|
|Background||SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.|
|Scientific References||PMID: 8663154 by Ravichandran V., et al. Identification of a novel syntaxin- and synaptobrevin/VAMP-binding protein, SNAP-23, expressed in non-neuronal tissues.|
PMID: 9070898 by Mollinedo F., et al. Identification of two isoforms of the vesicle-membrane fusion protein SNAP-23 in human neutrophils and HL-60 cells.
Our Boster Quality Guarantee for Anti-SNAP23 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat testis Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: SKOV Cell Lysate
IHC(P): Human Placenta Tissue
IHC(F): Human Placenta Tissue
ICC: K562 Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1774)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.