Anti-SNAP25 Antibody Picoband™

SNAP25 antibody

Boster Bio Anti-SNAP25 Antibody Picoband™ catalog # A01625. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: A01625
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-SNAP25 Antibody Picoband™

View all SNAP25 Antibodies

SKU/Catalog Number

A01625

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNAP25 Antibody Picoband™ catalog # A01625. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNAP25 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01625)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SNAP25 recombinant protein (Position:M1-L203).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01625 is reactive to SNAP25 in Human, Mouse, Rat

Applications

A01625 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

23.315kDa

Background of SNAP25

Synaptosome-associated protein of 25,000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SNAP25 (Source: Uniprot.org, NCBI)

Gene Name

SNAP25

Full Name

Synaptosomal-associated protein 25

Weight

23.315kDa

Superfamily

SNAP-25 family

Alternative Names

bA416N4.2; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP25; SNAP-25SNAP; SUP; Super protein; Synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa SNAP25 CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2 synaptosome associated protein 25 synaptosomal-associated protein 25|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein, 25kDa|synaptosome associated protein 25kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SNAP25, check out the SNAP25 Infographic

SNAP25 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SNAP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01625 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of spontaneous recurrent seizures on cognitive function via modulation of SNAREs expression

Disruption of glutamate neurotransmitter transmission is modulated by SNAP-25 in benzo[a]pyrene-induced neurotoxic effects

Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
A01625 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:250

Zhong ZQ, Xiang Y, Hu X, Wang YC, Zeng X, Wang XM, Xia QJ, Wang TH, Zhang X. Neural Regen Res. 2017 Jun;12(6):969-976. doi: 10.4103/1673-5374.208592. Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomo...

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16 Customer Q&As for Anti-SNAP25 Antibody Picoband™

Question

We have been able to see staining in mouse fetal brain temporal cortex. Are there any suggestions? Is anti-SNAP25 antibody supposed to stain fetal brain temporal cortex positively?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

According to literature fetal brain temporal cortex does express SNAP25. According to Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387

Boster Scientific Support

Answered: 2020-05-01

Question

Would anti-SNAP25 antibody A01625 work for WB with brain?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

According to the expression profile of brain, SNAP25 is highly expressed in brain. So, it is likely that anti-SNAP25 antibody A01625 will work for WB with brain.

Boster Scientific Support

Answered: 2020-04-22

Question

We ordered your anti-SNAP25 antibody for WB on skeletal muscle in a previous experiment. I am using mouse, and I plan to use the antibody for IHC-F next. I am looking for examining skeletal muscle as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-F?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

I have checked the website and datasheets of our anti-SNAP25 antibody and I see that A01625 has been tested on mouse in both WB and IHC-F. Thus A01625 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-F in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-F detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-13

Question

My question regarding product A01625, anti-SNAP25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01625 anti-SNAP25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-30

Question

We are currently using anti-SNAP25 antibody A01625 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

The anti-SNAP25 antibody (A01625) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-13

Question

Do you have a BSA free version of anti-SNAP25 antibody A01625 available?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 antibody A01625 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-13

Question

Would A01625 anti-SNAP25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

You can see on the product datasheet, A01625 anti-SNAP25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-18

Question

My colleagues were happy with the WB result of your anti-SNAP25 antibody. However we have observed positive staining in brain cytoplasm using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Based on literature, brain does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387

Boster Scientific Support

Answered: 2019-05-24

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SNAP25 antibody A01625. Let me know if you need anything else.

L. Evans

Verified customer

Asked: 2019-01-01

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-01

Question

See attached the WB image, lot number and protocol we used for brain using anti-SNAP25 antibody A01625. Please let me know if you require anything else.

B. Huang

Verified customer

Asked: 2018-11-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-13

Question

I see that the anti-SNAP25 antibody A01625 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-31

Question

Is a blocking peptide available for product anti-SNAP25 antibody (A01625)?

Verified Customer

Verified customer

Asked: 2017-12-15

Answer

We do provide the blocking peptide for product anti-SNAP25 antibody (A01625). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-15

Question

I was wanting to use your anti-SNAP25 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

You can see on the product datasheet, A01625 anti-SNAP25 antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-20

Question

I am looking for to test anti-SNAP25 antibody A01625 on human brain for research purposes, then I may be interested in using anti-SNAP25 antibody A01625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Anderson

Verified customer

Asked: 2016-12-06

Answer

The products we sell, including anti-SNAP25 antibody A01625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-12-06

Question

Is this A01625 anti-SNAP25 antibody reactive to the isotypes of SNAP25?

J. Kulkarni

Verified customer

Asked: 2013-06-04

Answer

The immunogen of A01625 anti-SNAP25 antibody is E. coli-derived human SNAP25 recombinant protein (Position:M1-L203). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-06-04

Question

We need using your anti-SNAP25 antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

A. Yang

Verified customer

Asked: 2013-01-10

Answer

We appreciate your inquiry. This A01625 anti-SNAP25 antibody is tested on human glioma tissue, hela whole cell lysate, rat brain tissue, mouse brain, u87 cells, u20s cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-01-10

Order DetailsPrice
A01625

100μg

$370
A01625-10ug

10μg sample (liquid)

$99
A01625-Biotin

100 μg Biotin conjugated

$570
A01625-Cy3

100 μg Cy3 conjugated

$570
A01625-Dylight488

100 μg Dylight488 conjugated

$570
A01625-Dylight550

100 μg Dylight550 conjugated

$570
A01625-Dylight594

100 μg Dylight594 conjugated

$570
A01625-FITC

100 μg FITC conjugated

$570
A01625-HRP

100 μg HRP conjugated

$570
A01625-APC

100 μg APC conjugated

$670
A01625-PE

100 μg PE conjugated

$670
A01625-carrier-free

Carrier Free

$370

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A01625
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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