Product Info Summary
SKU: | A01625 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SNAP25 Antibody Picoband™
SKU/Catalog Number
A01625
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNAP25 Antibody Picoband™ catalog # A01625. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAP25 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01625)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SNAP25 recombinant protein (Position:M1-L203).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01625 is reactive to SNAP25 in Human, Mouse, Rat
Applications
A01625 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
23.315kDa
Background of SNAP25
Synaptosome-associated protein of 25,000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (A01625).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (Catalog # A01625) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
Click image to see more details
Figure 2. IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. Flow Cytometry analysis of U87 cells using anti-SNAP25 antibody (A01625).
Overlay histogram showing U87 cells stained with A01625 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (A01625,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 7. Flow Cytometry analysis of U20S cells using anti-SNAP25 antibody (A01625).
Overlay histogram showing U20S cells stained with A01625 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (A01625,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 8. IF analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SNAP25 (Source: Uniprot.org, NCBI)
Gene Name
SNAP25
Full Name
Synaptosomal-associated protein 25
Weight
23.315kDa
Superfamily
SNAP-25 family
Alternative Names
bA416N4.2; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP25; SNAP-25SNAP; SUP; Super protein; Synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa SNAP25 CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2 synaptosome associated protein 25 synaptosomal-associated protein 25|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein, 25kDa|synaptosome associated protein 25kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNAP25, check out the SNAP25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNAP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNAP25 Antibody Picoband™ (A01625)
Hello CJ!
A01625 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of spontaneous recurrent seizures on cognitive function via modulation of SNAREs expression
Disruption of glutamate neurotransmitter transmission is modulated by SNAP-25 in benzo[a]pyrene-induced neurotoxic effects
Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
A01625 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:250
Zhong ZQ, Xiang Y, Hu X, Wang YC, Zeng X, Wang XM, Xia QJ, Wang TH, Zhang X. Neural Regen Res. 2017 Jun;12(6):969-976. doi: 10.4103/1673-5374.208592. Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomo...
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Customer Q&As
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16 Customer Q&As for Anti-SNAP25 Antibody Picoband™
Question
We have been able to see staining in mouse fetal brain temporal cortex. Are there any suggestions? Is anti-SNAP25 antibody supposed to stain fetal brain temporal cortex positively?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
According to literature fetal brain temporal cortex does express SNAP25. According to Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2020-05-01
Question
Would anti-SNAP25 antibody A01625 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
According to the expression profile of brain, SNAP25 is highly expressed in brain. So, it is likely that anti-SNAP25 antibody A01625 will work for WB with brain.
Boster Scientific Support
Answered: 2020-04-22
Question
We ordered your anti-SNAP25 antibody for WB on skeletal muscle in a previous experiment. I am using mouse, and I plan to use the antibody for IHC-F next. I am looking for examining skeletal muscle as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-F?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
I have checked the website and datasheets of our anti-SNAP25 antibody and I see that A01625 has been tested on mouse in both WB and IHC-F. Thus A01625 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-F in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-F detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-13
Question
My question regarding product A01625, anti-SNAP25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01625 anti-SNAP25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-SNAP25 antibody A01625 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-SNAP25 antibody (A01625) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
Question
Do you have a BSA free version of anti-SNAP25 antibody A01625 available?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 antibody A01625 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-13
Question
Would A01625 anti-SNAP25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can see on the product datasheet, A01625 anti-SNAP25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-18
Question
My colleagues were happy with the WB result of your anti-SNAP25 antibody. However we have observed positive staining in brain cytoplasm using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Based on literature, brain does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2019-05-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SNAP25 antibody A01625. Let me know if you need anything else.
L. Evans
Verified customer
Asked: 2019-01-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-01
Question
See attached the WB image, lot number and protocol we used for brain using anti-SNAP25 antibody A01625. Please let me know if you require anything else.
B. Huang
Verified customer
Asked: 2018-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-13
Question
I see that the anti-SNAP25 antibody A01625 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-31
Question
Is a blocking peptide available for product anti-SNAP25 antibody (A01625)?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
We do provide the blocking peptide for product anti-SNAP25 antibody (A01625). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-15
Question
I was wanting to use your anti-SNAP25 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
You can see on the product datasheet, A01625 anti-SNAP25 antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-20
Question
I am looking for to test anti-SNAP25 antibody A01625 on human brain for research purposes, then I may be interested in using anti-SNAP25 antibody A01625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Anderson
Verified customer
Asked: 2016-12-06
Answer
The products we sell, including anti-SNAP25 antibody A01625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-06
Question
Is this A01625 anti-SNAP25 antibody reactive to the isotypes of SNAP25?
J. Kulkarni
Verified customer
Asked: 2013-06-04
Answer
The immunogen of A01625 anti-SNAP25 antibody is E. coli-derived human SNAP25 recombinant protein (Position:M1-L203). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-04
Question
We need using your anti-SNAP25 antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
A. Yang
Verified customer
Asked: 2013-01-10
Answer
We appreciate your inquiry. This A01625 anti-SNAP25 antibody is tested on human glioma tissue, hela whole cell lysate, rat brain tissue, mouse brain, u87 cells, u20s cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-01-10