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- Table of Contents
SHH antibody (PA1072-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Anti-Sonic Hedgehog antibody, PA1072-1, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
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Anti-Sonic Hedgehog/SHH Antibody Picoband®
SHH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
SHH antibody was positively detected by WB in:
Rat Liver Tissue, Rat Intestine Tissue, HELA Cell, A549 Cell, SMMC Cell, MM231 Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SHH antibody was positively detected by IHC in:
Rat Brain Tissue, Human Mammary Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Tested
Recommend
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Sonic Hedgehog/SHH Antibody Picoband®
View all SHH Antibodies
View all SHH Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62-65 kDa
Calculated Molecular Weight
49.6 kDa
Background of SHH
The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Cite This Product
Anti-Sonic Hedgehog/SHH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1072-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Zebrafish
63%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SHH antibody
Immunohistochemistry (IHC) protocol for SHH antibody
Publications
Application:
Species:
Loxl3 Affects Palatal Shelf Elevation by Regulating Cell Proliferation and Collagen Deposition
Published: 2025-05-17
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Mouse
PMID: 40429955
LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts
Published: 2024-10-15
Journal: Molecular Medicine Reports
Application: IF,WB
Impact Factor: 3.40
Species: Human
PMID: 39422037
Astragaloside IV Treats Parkinson’s Disease by Regulating the Proliferation and Differentiation of NSCs through the SHH–Nurr1 Pathway
Published: 2024-09-03
Journal: Stem Cells International
Application: WB
Impact Factor: 3.80
Species: Rat
PMID: 39257865
Salidroside promotes angiogenesis after cerebral ischemia in mice through Shh signaling pathway
Published: 2024-04-20
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 6.90
Species: Mouse
PMID: 38643543
Rutaecarpine Ameliorates Murine N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway
Published: 2023-08-28
Journal: MOLECULES
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 37687125
Combined HIF-1α and SHH Up-Regulation Is a Potential Biomarker to Predict Poor Prognosis in Postoperative Hepatocellular Carcinoma
Published: 2022-06-29
Journal: JOURNAL OF INVESTIGATIVE SURGERY
Application: IHC
Impact Factor: 1.97
Species: Human
PMID: 35768071
A Noncanonical Hedgehog Signaling Exerts a Tumor-Promoting Effect on Pancreatic Cancer Cells Via Induction of Osteopontin Expression
Published: 2021-12-31
Journal: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
Application: IHC
Impact Factor: 3.10
Species: Human
PMID: 34978897
Sonic Hedgehog Expression Correlates With Distant Metastasis in Pancreatic Adenocarcinoma
Published: 2011-03-01
Journal: PANCREAS
Application: IHC
Impact Factor: 2.61
Species: Human
PMID: 20938369
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Product Promise
We promise Anti-Sonic Hedgehog/SHH Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1072-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026