|Product Name||Anti-Sonic Hedgehog/SHH Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Sonic Hedgehog/SHH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1072-1)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Sonic Hedgehog antibody, PA1072-1, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: MM231 Cell Lysate
Anti-Sonic Hedgehog antibody, PA1072-1, IHC(P)
IHC(P): Rat Brain Tissue
Anti-Sonic Hedgehog antibody, PA1072-1, IHC(P)
IHC(P): Human Mammary Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Sonic hedgehog protein|
|Tissue Specificity||Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.|
|Alternative Names||Sonic hedgehog protein;SHH;HHG-1;Sonic hedgehog protein N-product;Sonic hedgehog protein C-product;SHH;|
|Subcellular Localization||Sonic hedgehog protein C-product: Secreted, extracellular space . The C-terminal peptide diffuses from the cell. .|
|Molecular Weight||49607 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). .|
|Research Areas||Developmental Families, Domain Families, Embryogenesis, Embryonic Stem Cells, Epigenetics And Nuclear Signaling, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Neural Stem Cells, Oncoproteins/Suppressors, Organogenesis, Pathways And Processes, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Tumor Suppressors
*You can search these to find other products in these research areas.
|Background||The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,