Anti-Sonic Hedgehog/SHH Antibody
Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-Sonic Hedgehog/SHH Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Sonic Hedgehog/SHH Antibody
See all Sonic Hedgehog/Shh primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Sonic Hedgehog/SHH detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Sonic Hedgehog/SHH information: Molecular Weight: 49607 MW; Subcellular Localization: Sonic hedgehog protein C-product: Secreted, extracellular space . The C-terminal peptide diffuses from the cell; Tissue Specificity: Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.|
|Cite This Product||Anti-Sonic Hedgehog/SHH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1072-1)|
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|Anti-Sonic Hedgehog/SHH Antibody may replace the following items: sc 9024|sc 1194|sc 373779|sc 365112|sc 33943|sc 1195|sc 33942.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SHH (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6469|
|Species Of This Entry||Human|
|Protein Name||Sonic hedgehog protein|
|Alternative Names||Sonic Hedgehog/Shh|HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Palmitoylation; Acetylation; Acylation; Ribosylation; Dephosphorylation; Ubiquitination; Myristoylation; Demethylation; Carbamylation; Biotinylation; Glycosylation; Sumoylation; Deiodination; Reduction; Deacetylation; Oxidation|
|Subcellular Localization||[Sonic hedgehog protein N-product]: Cell membrane; Lipid-anchor. The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (PubMed:24522195). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides (PubMed:26875496, PubMed:24522195).|
|Gene Location||7q36.3, on Chromasome 7, gene sequence: NC_000007.14 (155799980..155812463, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SHH (Source: Uniprot.org, NCBI)
|Protein Function||Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).|
|Interacting Proteins||HHIP; PTCH1|
|Research Areas||Neuroscience; Sensory Systems; Stem Cell Signaling Pathway; Vision|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Nervousness; Cell Transformation, Neoplastic; Holoprosencephaly; Medulloblastoma; Congenital Abnormality; Polydactyly; Brain Neoplasms; Basal Cell Carcinoma; Malignant Paraganglionic Neoplasm; Cerebellar Neoplasms; Crest Syndrome; Neoplasm Metastasis; Carcinogenesis; Malignant Neoplasm Of Pancreas; Adenocarcinoma; Hypoplasia; Limb Deformities, Congenital|
|Related Pathways||Cell Proliferation; Limb Development; Pathogenesis; Cell Cycle; Localization; Cell Death; Neurogenesis; Regeneration; Cell Differentiation; Transport; Angiogenesis; Secretion; Gastrulation; Cell Growth; Lung Development; Forebrain Development; Brain Development; Axon Guidance; Cell Migration; Eye Development|
|Background||The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.|
|Scientific References||PMID: 7590746 by Marigo V., et al. Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog.|
PMID: 9593755 by Pepinsky R.B., et al. Identification of a palmitic acid-modified form of human Sonic hedgehog.
Our Boster Quality Guarantee for Anti-Sonic Hedgehog/SHH Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: MM231 Cell Lysate
IHC(P): Rat Brain Tissue
IHC(P): Human Mammary Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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