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Product Info Summary
| SKU: | A01665-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Sox-17 Antibody
SKU/Catalog Number
A01665-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Sox-17 Antibody catalog # A01665-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sox-17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01665-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Sox-17 . at AA range: 90-170
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01665-1 is reactive to SOX17 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01665-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from Jurkat cells, using Sox-17 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Sox-17 Antibody (A01665-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Sox-17 Antibody
Question
Do you have a BSA free version of anti-Sox-17 antibody A01665-1 available?
C. Miller
Verified customer
Asked: 2020-01-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Sox-17 antibody A01665-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-21
Question
Will anti-Sox-17 antibody A01665-1 work on horse WB with endometrium?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not validated anti-Sox-17 antibody A01665-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Sox-17 antibody A01665-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse endometrium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-Sox-17 antibody A01665-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
The anti-Sox-17 antibody (A01665-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-08
Question
I have a question about product A01665-1, anti-Sox-17 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01665-1 anti-Sox-17 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-01
Question
See attached the WB image, lot number and protocol we used for endometrium using anti-Sox-17 antibody A01665-1. Please let me know if you require anything else.
B. Gonzalez
Verified customer
Asked: 2018-08-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-24
Question
I was wanting to use your anti-Sox-17 antibody for WB for mouse endometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse endometrium identification?
B. Lewis
Verified customer
Asked: 2018-01-31
Answer
You can see on the product datasheet, A01665-1 anti-Sox-17 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse endometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-31
Question
I see that the anti-Sox-17 antibody A01665-1 works with WB, what is the protocol used to produce the result images on the product page?
K. Li
Verified customer
Asked: 2017-08-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-24
