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- Table of Contents
znf503 antibody (DZ12892) performs well in IF, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of nlz2 using anti-nlz2 antibody (DZ12892).
nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody (DZ12892) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody (DZ12892) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish nlz2 znf503 Antibody
znf503 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, WB |
Tested Applications
znf503 antibody was positively detected by WB in:
Zebrafish tissue, Zebrafish head tissue, Zebrafish body tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
znf503 antibody was positively detected by IF in:
zebrafish embryos tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5 μg/ml
Immunofluorescence
4 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish nlz2 znf503 Antibody
View all ZNF503 Antibodies
View all ZNF503 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Observed Molecular Weight
63 kDa
Cite This Product
Anti-Zebrafish nlz2 znf503 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ12892)
Protocols
Western Blot protocol for znf503 antibody
Immunofluorescence (IF) protocol for znf503 antibody
Review
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Submit A Review1 Review For Anti-Zebrafish nlz2 znf503 Antibody
Good Zebrafish Antibody for Nlz2--Jakub Famulski, University of Kentucky, Biology, Principal Investigator
Source: Biocompare.com
| SKU | DZ12892 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 24hpf |
| Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."
Jakub Famulski
Verified customer
Submitted 2019-12-06
Product Promise
We promise Anti-Zebrafish nlz2 znf503 Antibody performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
DZ12892
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 25, 2026