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Product Info Summary
| SKU: | PA1777 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband®
SKU/Catalog Number
PA1777
BA3738 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody catalog # PA1777. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1777)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1777 is reactive to SPTLC1 in Human, Mouse, Rat
Observed Molecular Weight
56 kDa
Calculated molecular weight
52.7 kDa
Background of SPTLC1
SPTLC1 (Serine palmitoyltransferase, long chain base subunit 1), also known as SPT1, LCB1, is a protein which in humans is encoded by the SPTLC1 gene. Dawkins et al. (2001)noted that the SPTLC1 gene maps to chromosome 9q22.1-q22.3. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1777 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human SiHa whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Western blot analysis of SPTLC1 using anti-SPTLC1 antibody (PA1777).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTLC1 antigen affinity purified polyclonal antibody (Catalog # PA1777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTLC1 at approximately 56 kDa. The expected band size for SPTLC1 is at 53 kDa.
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IHC analysis of SPTLC1 using antiSPTLC1 antibody (PA1777).
SPTLC1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SPTLC1 Antibody (PA1777) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband® (PA1777)
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Customer Q&As
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7 Customer Q&As for Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband®
Question
My question regarding product PA1777, anti-SPTLC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Roberts
Verified customer
Asked: 2020-04-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1777 anti-SPTLC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
Would anti-SPTLC1 antibody PA1777 work on canine WB with placenta?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Our lab technicians have not validated anti-SPTLC1 antibody PA1777 on canine. You can run a BLAST between canine and the immunogen sequence of anti-SPTLC1 antibody PA1777 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-14
Question
Does PA1777 anti-SPTLC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can see on the product datasheet, PA1777 anti-SPTLC1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-30
Question
Is there a BSA free version of anti-SPTLC1 antibody PA1777 available?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SPTLC1 antibody PA1777 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SPTLC1 antibody PA1777. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
Is this PA1777 anti-SPTLC1 antibody reactive to the isotypes of SPTLC1?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
The immunogen of PA1777 anti-SPTLC1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1(138-154aa FYGTFDVHLDLEDRLAK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-09
Question
I am looking for to test anti-SPTLC1 antibody PA1777 on human brain for research purposes, then I may be interested in using anti-SPTLC1 antibody PA1777 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Johnson
Verified customer
Asked: 2013-10-15
Answer
The products we sell, including anti-SPTLC1 antibody PA1777, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-15
