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Anti-SRY Antibody Picoband™
Boster Bio Anti-SRY Antibody Picoband™ catalog # A00614-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SRY Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00614-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence in the middle region of human SRY (90-130aa ISKQLGYQWKMLTEAEKWPFFQEAQKLQAMHREKYPNYKYR).
No cross reactivity with other proteins.
A00614-1 is reactive to SRY in Human
A00614-1 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SRY using anti-SRY antibody (A00614-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRY antigen affinity purified polyclonal antibody (Catalog # A00614-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRY at approximately 26KD. The expected band size for SRY is at 24KD.
Gene/Protein Information For SRY (Source: Uniprot.Org, NCBI)
Sex-determining region Y protein
essential protein for sex determination in human males; sex determining region Y; sex-determining region on Y; sex-determining region Y protein; TDFsex determining region protein; TDY; Testis-determining factor SRY SRXX1, SRXY1, TDF, TDY sex determining region Y sex-determining region Y protein|essential protein for sex determination in human males|sex-determining region on Y|testis-determining factor on Y|truncated sex-determining region Y protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SRY, check out the SRY Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SRY: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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