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STAT3 antibody (PB9511) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 27 Publications

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pb9511 stat3 primary antibodies fcm testing 6

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Flow Cytometry analysis of SiHa cells using anti-STAT3 antibody (PB9511).
Overlay histogram showing SiHa cells stained with PB9511 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT3 Antibody (PB9511, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used

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Anti-STAT3 Antibody Picoband®

STAT3 antibody

27 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

STAT3 antibody was positively detected by WB in:

human HEL whole cell lysates, human A549 whole cell lysates, human Hacat whole cell lysates, human U251 whole cell lysates, human K562 whole cell lysates, human SiHa whole cell lysates, human RT4 whole cell lysates, rat lung tissue lysates, rat heart tissue lysates, mouse lung tissue lysates, mouse heart tissue lysates, human Hela- WT whole cell, human Hela-STAT3 KO whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

STAT3 antibody was positively detected by IHC in:

human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

STAT3 antibody was positively detected by ICC/IF in:

A549 cell, U2OS cell

STAT3 antibody was positively detected by FC in:

SiHa cell, PC-12 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-STAT3 Antibody Picoband®
View all STAT3 Antibodies

Form

Lyophilized

Uniprot

P40763

Gene ID

6774

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human STAT3, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

88 kDa

Calculated Molecular Weight

88.1 kDa

Background of STAT3

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Cite This Product

Anti-STAT3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9511)

Protocols

Western Blot protocol for STAT3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for STAT3 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for STAT3 antibody

Review Protocol

Flow Cytometry (FC) protocol for STAT3 antibody

Review Protocol

Publications

Effect of Sun’s Bushen Huayu formula on M1/M2-associated markers and endometrial repair in a thin endometrium model

Published: 2026-08-17

Journal: Scientific Reports

Application: WB

Impact Factor: 4.90

Species: Rat

PMID: 42680819

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Free Secondary Antibody

Anti-STAT3 Antibody Picoband®

STAT3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Effect of Sun’s Bushen Huayu formula on M1/M2-associated markers and endometrial repair in a thin endometrium model

Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation

Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro

Drug screening identifies pyrrolidinedithiocarbamate ammonium ameliorating DSS-induced mouse ulcerative colitis via suppressing Th17 differentiation

In situ formed reactive oxygen species-responsive dipyridamole prodrug hydrogel: Spatiotemporal drug delivery for chemoimmunotherapy

α-linolenic acid ameliorates pentylenetetrazol-induced neuron apoptosis and neurological impairment in mice with seizures via down-regulating JAK2/STAT3 pathway

Langat virus inhibits the gp130/JAK/STAT signaling by reducing the gp130 protein level

Modified Biejia Jianwan decoction restrains PD-L1-mediated immune evasion through the HIF-1α/STAT3/NF-κB signaling pathway

Integration analysis using bioinformatics and experimental validation on the clinical and biological significance of TSLP in cancers

Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents

TIAM2 promotes proliferation and invasion of osteosarcoma cells by activating the JAK2/STAT3 signaling pathway

Overexpression of miR-125a-5p Inhibits Hepatocyte Proliferation through the STAT3 Regulation In Vivo and In Vitro

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling

Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis

High-Fat Diet-Related Obesity Promotes Urethane-Induced Lung Tumorigenesis in C57BL/6J Mice

JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis

MiR-296-3p may affect the proliferation and migration of non-small cell lung cancer cells via regulating RABL3

Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis

ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3–E1 cells through STAT3 activation

Celecoxib suppresses proliferation and metastasis of pancreatic cancer cells by down-regulating STAT3 / NF-kB and L1CAM activities

Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway

The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy

Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells

Effect of Iodine Excess on Th1, Th2, Th17, and Treg Cell Subpopulations in the Thyroid of NOD.H-2h4 Mice

Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuated Salmonella serovar typhimurium for prostate cancer treatment

Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer.

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

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