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Product Info Summary
| SKU: | MP01087 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody
SKU/Catalog Number
MP01087
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (Catalog # MP01087). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store STAT5 phospho Y694 Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01087)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 5F6.F1
Isotype
IgG1 kappa
Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
MP01087 is reactive to STAT5A in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
90.8 kDa
Background of STAT5A
Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5α and Stat5ß. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP01087 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000
IHC: 20-40 µg/ml
IF Microscopy: 1:50-1:1000
WB: 1:500 - 1:2,000
Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Validation Images & Assay Conditions
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Immunohistochemistry of Monoclonal Anti-Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti-Stat5pY694 at 20 µg/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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Western blot using Boster's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].
Specific Publications For Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (MP01087)
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