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Product Info Summary
| SKU: | A08661-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Stathmin-3 STMN3 Antibody
SKU/Catalog Number
A08661-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Stathmin-3 STMN3 Antibody catalog # A08661-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Stathmin-3 STMN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08661-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human STMN3. AA range:61-110
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08661-1 is reactive to STMN3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08661-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Stathmin-3 Polyclonal Antibody
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Western blot analysis of the lysates from K562 cells using STMN3 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Stathmin-3 STMN3 Antibody (A08661-1)
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Customer Q&As
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7 Customer Q&As for Anti-Stathmin-3 STMN3 Antibody
Question
Is this A08661-1 anti-Stathmin-3 antibody reactive to the isotypes of STMN3?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The immunogen of A08661-1 anti-Stathmin-3 antibody is Synthesized peptide derived from the Internal region of human Stathmin-3. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-06
Question
Is a blocking peptide available for product anti-Stathmin-3 antibody (A08661-1)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We do provide the blocking peptide for product anti-Stathmin-3 antibody (A08661-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-22
Question
Does anti-Stathmin-3 antibody A08661-1 work on pig WB with brain?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
Our lab technicians have not validated anti-Stathmin-3 antibody A08661-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Stathmin-3 antibody A08661-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-04
Question
We are currently using anti-Stathmin-3 antibody A08661-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
The anti-Stathmin-3 antibody (A08661-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-09
Question
I see that the anti-Stathmin-3 antibody A08661-1 works with WB, what is the protocol used to produce the result images on the product page?
M. Williams
Verified customer
Asked: 2017-07-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-11
Question
Does anti-Stathmin-3 antibody A08661-1 work for WB with adrenal gland?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
According to the expression profile of adrenal gland, STMN3 is highly expressed in adrenal gland. So, it is likely that anti-Stathmin-3 antibody A08661-1 will work for WB with adrenal gland.
Boster Scientific Support
Answered: 2017-05-18
Question
I have a question about product A08661-1, anti-Stathmin-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Parker
Verified customer
Asked: 2017-04-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08661-1 anti-Stathmin-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-04-27
