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Product Info Summary
| SKU: | M02224-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10]
SKU/Catalog Number
M02224-1
Size
100 µl
Description
Boster Bio Anti-STK3 mouse monoclonal antibody, clone OTI4G10 (formerly 4G10). Catalog# M02224-1. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02224-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4G10
Isotype
IgG2b
Immunogen
Full-length protein expressed in 293T cell transfected with human STK3 expression vector
Reactive Species
M02224-1 is reactive to STK3 in Dog, Human, Monkey, Mouse, Rat
Observed Molecular Weight
56 kDa
Calculated molecular weight
56.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02224-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
Positive Control
WB: human 293T whole cell, human MCF-7 whole cell, human Jurkat whole cell, rat RH35 whole cell, rat C6 whole cell, mouse RAW264.7 whole cell, mouse NIH/3T3 whole cell
IHC:
human pancreas cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of STK3 using anti-STK3 antibody (M02224-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STK3 antigen affinity purified monoclonal antibody (M02224-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STK3 at approximately 56 kDa. The expected band size for STK3 is at 56 kDa.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK3.
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IHC analysis of STK3 using anti-STK3 antibody (M02224-1).
STK3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 mouse anti-STK3 Antibody (M02224-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Specific Publications For Anti-STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10] (M02224-1)
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