|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-p27Kip1 Antibody (Monoclonal, DCS-72)|
|Description||Mouse IgG monoclonal antibody for p27Kip1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-p27Kip1 Antibody (Monoclonal, DCS-72) DCS-72 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1076)|
|Replacement Item||This antibody may replace the following items: sc-12939-R|sc-130603|sc-16324|sc-16324-R|sc-1641|sc-393305|sc-393380|sc-527|sc-528|sc-53871|sc-53906|sc-56338|sc-56454|sc-71813|sc-776 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||recombinant rodent p27Kip1 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase inhibitor 1B|
|Molecular Weight||22139 MW|
|Protein Function||CDKN1B: Important regulator of cell cycle progression. Involvedin G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2complexes. Forms a complex with cyclin type D-CDK4 complexes andis involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or anactivator of cyclin type D-CDK4 complexes depending on itsphosphorylation state and/or stoichometry.|
|Tissue Specificity||CDKN1B: Expressed in all tissues tested. Highestlevels in skeletal muscle, lowest in liver and kidney.|
|Sequence Similarities||Belongs to the CDI family.|
|Subcellular Localization||CDKN1B: Nucleus. Cytoplasm. Endosome Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates tothe cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results intranslocation to the cytoplasm and cell cycle progression.Phosphorylation on Ser-10 facilitates nuclear export. Translocatesto the nucleus on phosphorylation of Tyr-88 and Tyr-89.Colocalizes at the endosome with SNX6; this leads to lysosomaldegradation (By similarity).
Cdkn1b: Nucleus. Cytoplasm. Endosome. Note: Nuclearand cytoplasmic in quiescent cells. AKT- or RSK-mediatedphosphorylation on Thr-197, binds 14-3-3, translocates to thecytoplasm and promotes cell cycle progression. Mitogen-activatedUHMK1 phosphorylation on Ser-10 also results in translocation tothe cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus onphosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizesat the endosome with SNX6; this leads to lysosomal degradation.
|Alternative Names||Cyclin dependent kinase inhibitor ;Cyclin kinase inhibitor ;Cyclin-dependent kinase inhibitor 1B ;P27 kip1 ;P27kip1 ;Protein Cdkn1b ;Cdkn1b ;p27Kip1 ;rCG_29991 ;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|cip / kip| epigenetics and nuclear signaling|cip/kip| cancer||