Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: MA1076
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: ICC, WB

Product Name

Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

See all p27/Kip1 products

SKU/Catalog Number

MA1076

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1076)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-72

Isotype

Mouse IgG1

Immunogen

recombinant rodent p27Kip1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1076 is reactive to CDKN1B in Human, Mouse, Rat

Applications

MA1076 is guaranteed for ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 2-4μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For CDKN1B (Source: Uniprot.Org, NCBI)

Uniprot ID

P46527

Gene ID

1027

Gene Name

CDKN1B

Full Name

Cyclin-dependent kinase inhibitor 1B

Weight

22.073kDa

Superfamily

CDI family

Alternative Names

CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDKN1B, check out the CDKN1B Infographic

CDKN1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDKN1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1076 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...

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7 Customer Q&As for Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

Question

Is a blocking peptide available for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076)?

G. Taylor

Verified customer

Asked: 2020-02-21

Answer

We do provide the blocking peptide for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-21

Question

See attached the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-02

Question

I have a question about product MA1076, anti-p27Kip1 antibody (Monoclonal, DCS-72). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1076 anti-p27Kip1 antibody (Monoclonal, DCS-72), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-20

Question

you antibody to test anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 on rat kidney for research purposes, then I may be interested in using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

The products we sell, including anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-24

Question

Do you have a BSA free version of anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 available?

Verified Customer

Verified customer

Asked: 2017-05-25

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-25

Question

I see that the anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 works with WB, what is the protocol used to produce the result images on the product page?

Z. Wu

Verified customer

Asked: 2015-09-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-09-28

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Let me know if you need anything else.

T. Roberts

Verified customer

Asked: 2014-12-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-12-29

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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MA1076
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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