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Product Info Summary
| SKU: | MA1076 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)
SKU/Catalog Number
MA1076
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1076)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
DCS-72
Isotype
Mouse IgG1
Immunogen
recombinant rodent p27Kip1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1076 is reactive to CDKN1B in Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
22139 MW
Background of p27/Kip1
P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1076 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 2-4μg/ml, Human, mouse, rat
Positive Control
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)
Gene Name
CDKN1B
Full Name
Cyclin-dependent kinase inhibitor 1B
Weight
22139 MW
Superfamily
CDI family
Alternative Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1B, check out the CDKN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) (MA1076)
Hello CJ!
MA1076 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)
Question
Is a blocking peptide available for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076)?
G. Taylor
Verified customer
Asked: 2020-02-21
Answer
We do provide the blocking peptide for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-21
Question
See attached the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-02
Question
I have a question about product MA1076, anti-p27Kip1 antibody (Monoclonal, DCS-72). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1076 anti-p27Kip1 antibody (Monoclonal, DCS-72), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-20
Question
you antibody to test anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 on rat kidney for research purposes, then I may be interested in using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
The products we sell, including anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-24
Question
Do you have a BSA free version of anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 available?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-25
Question
I see that the anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2015-09-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-09-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Let me know if you need anything else.
T. Roberts
Verified customer
Asked: 2014-12-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-29
