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Product Info Summary
| SKU: | MA1076 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)
SKU/Catalog Number
MA1076
BM1594 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1076)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
DCS-72
Isotype
Mouse IgG1
Immunogen
recombinant rodent p27Kip1 protein.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1076 is reactive to CDKN1B in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Background of CDKN1B
P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1076 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 2-4μg/ml, Human, mouse, rat
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) (MA1076)
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Customer Q&As
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7 Customer Q&As for Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)
Question
Is a blocking peptide available for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076)?
G. Taylor
Verified customer
Asked: 2020-02-21
Answer
We do provide the blocking peptide for product anti-p27Kip1 antibody (Monoclonal, DCS-72) (MA1076). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-21
Question
See attached the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-02
Question
I have a question about product MA1076, anti-p27Kip1 antibody (Monoclonal, DCS-72). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1076 anti-p27Kip1 antibody (Monoclonal, DCS-72), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-20
Question
you antibody to test anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 on rat kidney for research purposes, then I may be interested in using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
The products we sell, including anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-24
Question
Do you have a BSA free version of anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 available?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-25
Question
I see that the anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2015-09-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-09-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-p27Kip1 antibody (Monoclonal, DCS-72) MA1076. Let me know if you need anything else.
T. Roberts
Verified customer
Asked: 2014-12-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-29
