Anti-Tamalin GRASP Antibody

Product Name

Anti-Tamalin GRASP Antibody

View all GRASP Antibodies

SKU/Catalog Number

A11078

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Tamalin GRASP Antibody (Catalog # A11078). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Tamalin GRASP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11078)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein.

Reactive Species

A11078 is reactive to GRASP in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.3 kDa

Background of GRASP

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs).  The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs.  The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins.  It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11078 is guaranteed for ELISA, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:20,000
IP: 1μg
WB: 1:500 - 1:3,000
This affinity purified antibody has been tested for use in ELISA, western blotting, and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to Tamalin protein by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information GRASP

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