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Product Info Summary
| SKU: | A00445-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-TANK Antibody
SKU/Catalog Number
A00445-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TANK Antibody catalog # A00445-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TANK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00445-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TANK. AA range:171-220
Reactive Species
A00445-1 is reactive to TANK in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
47.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00445-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Western Blot analysis of HT-29 cells using TANK Polyclonal Antibody
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Western blot analysis of lysates from HT29 cells, using I-TRAF Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TANK Antibody (A00445-1)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-TANK Antibody
Question
Will anti-TANK antibody A00445-1 work on monkey WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Our lab technicians have not validated anti-TANK antibody A00445-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TANK antibody A00445-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-04
Question
Would anti-TANK antibody A00445-1 work for WB with bone marrow?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
According to the expression profile of bone marrow, TANK is highly expressed in bone marrow. So, it is likely that anti-TANK antibody A00445-1 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2018-10-18
Question
Can you help my question with product A00445-1, anti-TANK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Taylor
Verified customer
Asked: 2017-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00445-1 anti-TANK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-30
