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- Table of Contents
1 Q&As
Facts about TRAF family member-associated NF-kappa-B activator.
Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed:25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 at a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).
Human | |
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Gene Name: | TANK |
Uniprot: | Q92844 |
Entrez: | 10010 |
Belongs to: |
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No superfamily |
ITRAF; I-TRAF; I-TRAFTRAF2; TANK; TRAF family member-associated NF-kappa-B activator; TRAF family member-associated NFKB activator; TRAF-interacting protein
Mass (kDA):
47.816 kDA
Human | |
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Location: | 2q24.2 |
Sequence: | 2; NC_000002.12 (161136955..161236230) |
Ubiquitous.
Cytoplasm.
Boster Bio: Best Uses of the Tank Marker is a well-known method of detecting colorimetric chemiluminescence that is used to detect fatty acids within urine. Contrary to conventional dyes, this marker has no negative side effects, and is highly effective in a variety of medical tests. Its high-qualityand easy-to-use design permits flexibility and versatility in a variety of labs.
ECL can detect tiny amounts of protein in cells, tissues and other samples. The long half-life of the emission and sensitivity to tiny amounts of target proteins make it a great option for detecting proteins in complex samples. This technique can detect small amounts target proteins with low Femtograms. Enhanced Chemiluminescence detection (ECL) using the TANK Marker can increase the intensityther samsignal and also allows to reimaging without losing the original signal.
Utilizing fluorescently labeled antibodies in the chemiluminescence detection process is a simple and quick procedure that doesn't require any complicated creation her samsubstrate. These antibodies are compatible with a majoritytherwestern-blots. They can also be used with any horseradish/peroxidase-conjugate. ECL is an chemistry used to detect proteins. It is based on emission light at 428nm. This can be captured using a digital camera or X-ray film.
One of the most effective methods to detect toxic gasses in a workplace is to use a colorimetric tube. The colorimetric tube is called stain tubes, or gas detectors. These devices use graduated glass tubes that are filled with chemical reagents to measure the levels of gas contamination. After exposure to a specific gas, the tubes cause changes in color. To monitor continuously, a colorimetric tube can be used in conjunction with an electronic hand pump.
Two enzymes are utilized in an experiment that tests color: AP and HRP. AP has lower catalytic activity than HRP However, its product fades faster. HRP has a lower rate of activity due to its reaction to oxidation. AP, on the other it is not limited by the oxidation reaction, and has a linear activities for longer durations of time. A longer duration of incubation can result in more background noise and lower signal-to-noise ratio.
It is simple to do an in-house colorimetric blot. A secondary antibody conjugated to an enzyme reporter reacts to a chromogenic substratum to produce a visually discernable signal. The result is a colored band that can be seen by the human eye. Additionally the cost of colorimetric blotting is lower when compared to chemiluminescent-based immunoassays.
DAB is also referred to as Enhanced Chemiluminescence. Both of them use HRP-conjugated antibodies. You can purchase a kit that includes both kinds of reagents. This is convenient and reliable. The kits decrease the risk of being exposed chemicals. They are readily available and can be purchased from a variety of manufacturers.
Steven Boster's TANK marker which was first developed in 1993 it is a high-affinity primary antibody used in ELISA tests. Based on proprietary trade secrets, Boster antibodies are highly used in scientific research and widely trusted in the research community. Utilizing Boster antibodies researchers can create high-sensitivity ELISA kits for analyzing protein and lipid samples. Boster's TANK marker is extremely versatile and accurate for various types of protein analysis, is a fantastic tool.
PMID: 8710854 by Rothe M., et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF- mediated signal transduction.
PMID: 8855313 by Kaye K.M., et al. Tumor necrosis factor receptor associated factor 2 is a mediator of NF-kappa B activation by latent infection membrane protein 1, the Epstein-Barr virus transforming protein.