Anti-TAPA1/CD81 Antibody Picoband®

Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse raw264.7 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.

IHC analysis of TAPA1 using anti-TAPA1 antibody (A01281-2).
TAPA1 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TAPA1 Antibody (A01281-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Identification of hAMSC-Exos ( A ) The morphology of hAMSC-Exos was observed using transmission electron microscopy. Scale bar = 100 nm. ( B ) The hAMSC-Exos particle size distribution was detected using NTA. ( C ) Western blot was used to detect the expression of hAMSC-Exos surface proteins CD9, CD63 and CD81
Index in PubMed under a CC BY license. PMID: 40329179

Western blot analysis of specific protein expression ( n = 3). ( A ) Colored bands of GFAP, LRP6, THBS1 and CD81; ( B ) Expression levels of LRP6, THBS1 and CD81 gradually decreased with the increase of the stretch amplitude, while the expression level of GFAP gradually increased with the increase of the stretch amplitude. Error bars indicate standard error of the mean (SEM); * P < 0.05 and ** P < 0.01 indicate statistical significance
Index in PubMed under a CC BY license. PMID: 36114477

Flow Cytometry analysis of HL-60 cells using anti-TAPA1 antibody (A01281-2).
Overlay histogram showing HL-60 cells stained with A01281-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of U2OS cells using anti-TAPA1 antibody (A01281-2).
Overlay histogram showing U2OS cells stained with A01281-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.