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Product Info Summary
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
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Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat IL4 in cell culture supernatants and serum. Sensitivity: 1 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0406)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: C25-S147
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0406 is reactive to IL4 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EZ0406 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0406-CA||Mouse anti- rat il4 monoclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0406-DA||Biotinylated goat anti- rat il4 polyclonal antibody (Detection Antibody)||500 µl, 11.4 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0406-ST||Lyophilized recombinant rat il4 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat IL-4 EZ Set ELISA Kit standard curve
Gene/Protein Information For IL4 (Source: Uniprot.Org, NCBI)
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra Il4|Il4e12|interleukin 4|interleukin-4|B-cell IGG differentiation factor|B-cell growth factor 1|B-cell stimulatory factor 1|BSF-1|IL-4|lymphocyte stimulatory factor 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL4, check out the IL4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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