Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat IL4 in cell culture supernatants and serum. Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ0406
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Rat
Application: ELISA

Product Name

Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL-4 products

SKU/Catalog Number

EZ0406

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat IL4 in cell culture supernatants and serum. Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0406)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: C25-S147

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0406 is reactive to IL4 in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EZ0406 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0406-CA Mouse anti- rat il4 monoclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0406-DA Biotinylated goat anti- rat il4 polyclonal antibody (Detection Antibody) 500 µl, 11.4 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0406-ST Lyophilized recombinant rat il4 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0680.1070.1430.2290.4700.7931.5612.163

Data Example Images

Gene/Protein Information For IL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20096

Gene ID

287287

Gene Name

IL4

Full Name

Interleukin-4

Weight

16.247kDa

Superfamily

IL-4/IL-13 family

Alternative Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra Il4|Il4e12|interleukin 4|interleukin-4|B-cell IGG differentiation factor|B-cell growth factor 1|B-cell stimulatory factor 1|BSF-1|IL-4|lymphocyte stimulatory factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL4, check out the IL4 Infographic

IL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EZ0406

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

1 Customer Q&As for Rat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

I have a serum samples, can this Rat IL-4 ELISA Kit EZ-Set be run neat without dilution?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

The serum and plasma samples might be run undiluted but we do not recommend doing that since assay diluent needs to be present in every samples but testing undiluted could increase the risk of matrix effect. Matrix effects happen when some element of the sample affects immunoreactivity of the target molecule, perhaps by sequestering binding sites. Auto-antibodies, binding proteins, albumin, etc. might be the possible culprits, but it

Boster Scientific Support

Answered: 2018-07-05

$220

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EZ0406
$220.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.