Rabbit IgG polyclonal antibody for Microtubule-associated protein tau(MAPT) detection. Tested with WB in Human;Mouse;Rat. Cited in 10 publication(s).
Anti-Tau/MAPT Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Tau/MAPT Antibody
See all Tau primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Tau/MAPT detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Tau/MAPT information: Molecular Weight: 78928 MW; Subcellular Localization: Cytoplasm, cytosol . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell projection, axon . Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components; Tissue Specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.|
|Cite This Product||Anti-Tau/MAPT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1482)|
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|Anti-Tau/MAPT Antibody may replace the following items: sc 101556|sc 101555|sc 28976|sc 19546|sc 19547.|
|Reactivity/Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Tau(29-44aa YTMHQDQEGDTDAGLK), different from the related mouse and rat sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MAPT (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4137|
|Species Of This Entry||Human|
|Protein Name||Microtubule-associated protein tau|
|Alternative Names||Tau|DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MGC138549; microtubule-associated protein tau; MSTD; MSTDMAPTL; MTBT1; MTBT1Neurofibrillary tangle protein; MTBT2; Neurofibrillary tangle protein; PHF-tau; PPND; Tau; TAUPaired helical filament-tau|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Ubiquitination; Reduction; Acetylation; Methylation; Sulphation; Deamination; Amidation; Gamma-cleavage; Biotinylation; Deacetylation; Glycosylation|
|Subcellular Localization||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.|
|Gene Location||17q21.31, on Chromasome 17, gene sequence: NC_000017.11 (45894382..46028334)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MAPT (Source: Uniprot.org, NCBI)
|Protein Function||Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.|
|Tissue Specificity||Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.|
|Interacting Proteins||AKT1; ANXA2; ANXA6; APOE; APP; DCTN1; DDX6; FKBP5; FYN; GSK3B; HSP90AB1; HSPA8; HTRA1; LRRK2; MAPRE3; MARK2; PRNP; RPL28; SFN; SLC1A2; SNCA; STUB1; YWHAZ|
|Research Areas||Alzheimers Research; Apoptosis; Cell Biology; Cellular Markers; Cytokine Research; MAP Kinase Signaling; Neurodegeneration; Neuroscience; Phospho-Specific; Stem Cell Markers|
|Related Diseases||Alzheimer's Disease; Dementia; Frontotemporal Dementia; Neurodegenerative Disorders; Tauopathies; Neurofibrillary Tangles; Secondary Parkinson Disease; Nerve Degeneration; Parkinson Disease; Progressive Supranuclear Palsy; Supranuclear Palsy; Frontotemporal Lobar Degeneration; Atrophy; Impaired Cognition; Corticobasal Degeneration; Pick Disease Of The Brain; Plaque, Amyloid; Cognition Disorders; Senile Plaques|
|Related Pathways||Pathogenesis; Hyperphosphorylation; Aging; Transport; Cell Death; Localization; Proteolysis; Secretion; Cognition; Cell Cycle; Mrna Splicing; Translation; Autophagy; Protein Phosphorylation; Neurogenesis; Methylation; Dna Methylation; Mitosis; Locomotion; Rna Interference|
|Background||MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease.|
|Scientific References||PMID: 3131773 by Goedert M., et al. Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein tau.|
PMID: 2498079 by Goedert M., et al. Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein tau containing four tandem repeats: differential expression of tau protein mRNAs in human brain.
Our Boster Quality Guarantee for Anti-Tau/MAPT Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti (PA1482) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 79KD
Observed bind size: 79KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 10
|Tu Q, Pang L, Chen Y, Zhang Y, Zhang R, Lu B, Wang J. Analyst. 2014 Jan 7;139(1):105-15. Doi: 10.1039/C3An01796F. Epub 2013 Oct 25. Effects Of Surface Charges Of Graphene Oxide On Neuronal Outgrowth And Branching.||PubMed ID 24162459|
|Tan Wf, Cao Xz, Wang Jk, Lv Hw, Wu By, Ma H. Clin Exp Pharmacol Physiol. 2010 Oct;37(10):1010-5. Doi: 10.1111/J.1440-1681.2010.05433.X. Protective Effects Of Lithium Treatment For Spatial Memory Deficits Induced By Tau Hyperphosphorylation In Sple...||PubMed ID 20659131|
|Zhu Yf, Li Xh, Yuan Zp, Li Cy, Tian Rb, Jia W, Xiao Zp. Eur J Pharmacol. 2015 Sep 5;762:239-46. Doi: 10.1016/J.Ejphar.2015.06.002. Epub 2015 Jun 3. Allicin Improves Endoplasmic Reticulum Stress-Related Cognitive Deficits Via Perk/Nrf2 Antioxidativ...||PubMed ID 26049013|
|Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.||PubMed ID 25317137|
|Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...||PubMed ID 28934273|
|Chen C, Gu J, Basurto-Islas G, Jin N, Wu F, Gong CX, Iqbal K, Liu F. Sci Rep. 2017 Oct 18;7(1):13478. doi: 10.1038/s41598-017-13791-5. Up-regulation of casein kinase 1ε is involved in tau pathogenesis in Alzheimer’s disease||PubMed ID 29044200|
|Panax notoginsenoside Rb1 ameliorates Alzheimer?s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression||PubMed ID 24137274|
|The expression of insulin-like growth factor-1 in senior patients with diabetes and dementia||PubMed ID 28123476|
|Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment||PubMed ID 29138799|
|Micro-RNA-137 Inhibits Tau Hyperphosphorylation in Alzheimer's Disease and Targets the CACNA1C Gene in Transgenic Mice and Human Neuroblastoma %u2026||PubMed ID 30102687|
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Note: sample size takes longer to process, average 5-7 days.
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