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- Table of Contents
MAPT antibody (A00097-3) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3).
Tau/MAPT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Tau/MAPT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Tau/MAPT Antibody Picoband®
MAPT antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
MAPT antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MAPT antibody was positively detected by IHC in:
human brain tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MAPT antibody was positively detected by ICC/IF in:
T-47D cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Tau/MAPT Antibody Picoband®
View all MAPT Antibodies
View all MAPT Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tau/MAPT recombinant protein (Position: M1-L322).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50-70 kDa
Calculated Molecular Weight
78.9 kDa
Background of MAPT
MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease.
Cite This Product
Anti-Tau/MAPT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00097-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
91%
High Homology
Dog
73%
Moderate Homology
Cow
68%
Low homology
Sheep
67%
Low homology
Rat
62%
Low homology
Rabbit
61%
Low homology
Mouse
61%
Low homology
Zebrafish
21%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MAPT antibody
Immunohistochemistry (IHC) protocol for MAPT antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MAPT antibody
Publications
Application:
Species:
Bushen Huoxue Acupuncture alleviates alzheimer’s disease progression via the E3 ubiquitin ligase SMURF2‑mediated ubiquitination of LAPTM5
Published: 2026-06-29
Journal: METABOLIC BRAIN DISEASE
Application: IF,IHC
Impact Factor: 3.80
Species: Mouse
PMID: 42371177
IL-1β neutralization ameliorates cognitive deficits and tau pathology in a mouse model of Alzheimer's disease with hyperhomocysteinemia
Published: 2026-03-15
Journal: EXPERIMENTAL NEUROLOGY
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 41845989
Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease
Published: 2012-12-12
Journal: Neural Regeneration Research
Application: IHC
Impact Factor: 0.22
Species: Rat
PMID: 25317137
Spatiotemporally Controlled and Multifactor Involved Assay of Neuronal Compartment Regeneration after Chemical Injury in an Integrated Microfluidics
Published: 2012-07-26
Journal: ANALYTICAL CHEMISTRY
Application: ICC
Impact Factor: 5.86
Species: Rat
PMID: 22793989
The effect of acetylcholine-like biomimetic polymers on neuronal growth
Published: 2011-02-06
Journal: BIOMATERIALS
Application: IHC
Impact Factor: 7.88
Species: Rat
PMID: 21303719
Simultaneous expression of Oct4 and genes of three germ layers in single cell-derived multipotent adult progenitor cells
Published: 2008-03-13
Journal: ANNALS OF HEMATOLOGY
Application: IF
Impact Factor: 2.34
Species: Rat
PMID: 18338169
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Product Promise
We promise Anti-Tau/MAPT Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00097-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026