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Product Info Summary
| SKU: | M00097 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Tau MAPT Rabbit Monoclonal Antibody
SKU/Catalog Number
M00097
BM4682 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tau MAPT Rabbit Monoclonal Antibody catalog # M00097. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tau MAPT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00097)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HAB-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tau
Reactive Species
M00097 is reactive to MAPT in Human, Mouse, Rat
Observed Molecular Weight
50-80 kDa
Calculated molecular weight
78.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00097 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
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Western blot analysis of Tau expression in Mouse brain lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of T-47D cells, using Tau Antibody.
Specific Publications For Anti-Tau MAPT Rabbit Monoclonal Antibody (M00097)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Tau MAPT Rabbit Monoclonal Antibody
Question
What is the difference between Anti-Tau Rabbit Monoclonal antibodies M00097 and M00097-1?
Verified customer
Asked: 2019-10-10
Answer
The Anti-Tau MAPT Rabbit Monoclonal Antibody (M00097) and Anti-Tau MAPT Rabbit Monoclonal Antibody (M00097-1) differ in the immunogen sequence and application.
Boster Scientific Support
Answered: 2019-10-11
Question
We ordered your anti-Tau Rabbit Monoclonal antibody for IF on fetal brain last year. I am using rat, and I plan to use the antibody for WB next. I am interested in examining fetal brain as well as leukemic t-cell in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
I looked at the website and datasheets of our anti-Tau Rabbit Monoclonal antibody and it seems that M00097 has been validated on rat in both IF and WB. Thus M00097 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-09
Question
We have seen staining in human parietal lobe. Are there any suggestions? Is anti-Tau Rabbit Monoclonal antibody supposed to stain parietal lobe positively?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
From literature parietal lobe does express MAPT. From Uniprot.org, MAPT is expressed in parietal lobe, brain, fetal brain, fetal brain cortex, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-11
Question
My boss were satisfied with the WB result of your anti-Tau Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia cytoplasm using this antibody. Is that expected? Could you tell me where is MAPT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
From what I have seen in literature, cervix carcinoma erythroleukemia does express MAPT. Generally MAPT expresses in cytoplasm, cytosol. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-01-30
Question
We are currently using anti-Tau Rabbit Monoclonal antibody M00097 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
D. Li
Verified customer
Asked: 2014-03-25
Answer
The anti-Tau Rabbit Monoclonal antibody (M00097) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-25
