Anti-TGF alpha/TGFA Antibody


SKU PA1360
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-TGF alpha/TGFA Antibody
SKU/Catalog Number PA1360
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Protransforming growth factor alpha(TGFA) detection. Tested with WB in Human.
Cite This Product Anti-TGF alpha/TGFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1360)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human TGF alpha(73-89aa CHSGYVGARCEHADLLA), different from the related mouse and rat sequences by one amino acid.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-TGF alpha antibody, PA1360, Western blotting
All lanes: Anti TGF alpha (PA1360) at 0.5ug/ml
Lane 1: Recombinant Human TGF a Protein 10ng
Lane 2: Recombinant Human TGF a Protein 5ng
Lane 3: Recombinant Human TGF a Protein 2.5ng
Predicted bind size: 6KD
Observed bind size: 6KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01135
Gene Name TGFA
Protein Name Protransforming growth factor alpha
Tissue Specificity Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .
Alternative Names Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA;
Subcellular Localization Transforming growth factor alpha: Secreted, extracellular space.
Molecular Weight 17006 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Research Areas Human

*You can search these to find other products in these research areas.
Background Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGFalpha stimulates neural cell proliferation in the adult injured brain. TGFalpha was cited in the 2001 NIH Stem Cell report to the U.S. Congress as promising evidence for the ability of adult stem cells to restore function in neurodegenerative disorders.

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Polyclonal antibody for TGF ALPHA/TGFA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. TGF ALPHA/TGFA information: Molecular Weight: 17006 MW; Subcellular Localization: Transforming growth factor alpha: Secreted, extracellular space; Tissue Specificity: Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
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In stock
Order Product
PA1360
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Ruan Gp, Xu F, Li Za, Zhu Gx, Pang Rq, Wang Jx, Cai Xm, He J, Yao X, Ruan Gh, Xu Xm, Pan Xh. Plos One. 2013 Dec 18;8(12):E83507. Doi: 10.1371/Journal.Pone.0083507. Ecollection 2013. Induced Autologous Stem Cell Transplantation For Treatment Of Rab...
Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: One other very common name is tgf-alpha antibody
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