Anti-TRAF2 Antibody

TRAF-2 antibody

Boster Bio Anti-TRAF2 Antibody catalog # PA2007. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2007
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-TRAF2 Antibody

View all TRAF-2 Antibodies

SKU/Catalog Number

PA2007

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAF2 Antibody catalog # PA2007. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2007)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TRAF2, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2007 is reactive to TRAF2 in Human, Mouse, Rat

Applications

PA2007 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

55.859kDa

Background of TRAF-2

TRAF2 (TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al. (1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al. (2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For TRAF2 (Source: Uniprot.org, NCBI)

Gene Name

TRAF2

Full Name

TNF receptor-associated factor 2

Weight

55.859kDa

Superfamily

TNF receptor-associated factor family

Alternative Names

E3 ubiquitin-protein ligase TRAF2; EC 6.3.2.-; MGC:45012; TNF receptor-associated factor 2; TRAF2; TRAF-2; TRAP3; TRAP3TRAP; tumor necrosis factor type 2 receptor associated protein 3; Tumor necrosis factor type 2 receptor-associated protein 3 TRAF2 MGC:45012, RNF117, TRAP, TRAP3 TNF receptor associated factor 2 TNF receptor-associated factor 2|E3 ubiquitin-protein ligase TRAF2|RING-type E3 ubiquitin transferase TRAF2|tumor necrosis factor type 2 receptor associated protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAF2, check out the TRAF2 Infographic

TRAF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-TRAF2 Antibody

Question

Would anti-TRAF2 antibody PA2007 work for WB with stomach uterus?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

According to the expression profile of stomach uterus, TRAF2 is highly expressed in stomach uterus. So, it is likely that anti-TRAF2 antibody PA2007 will work for WB with stomach uterus.

Boster Scientific Support

Answered: 2019-08-30

Question

Will anti-TRAF2 antibody PA2007 work on bovine IHC with brain?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

Our lab technicians have not validated anti-TRAF2 antibody PA2007 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-TRAF2 antibody PA2007 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-23

Question

I see that the anti-TRAF2 antibody PA2007 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-31

Question

Is a blocking peptide available for product anti-TRAF2 antibody (PA2007)?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

We do provide the blocking peptide for product anti-TRAF2 antibody (PA2007). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-05

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach uterus using anti-TRAF2 antibody PA2007. Let me know if you need anything else.

J. Collins

Verified customer

Asked: 2018-12-21

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-21

Question

I have a question about product PA2007, anti-TRAF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Bhatt

Verified customer

Asked: 2014-03-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2007 anti-TRAF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-03-17

Order DetailsPrice
PA2007

100μg

$370
PA2007-10ug

10μg sample (liquid)

$99
PA2007-Biotin

100 μg Biotin conjugated

$570
PA2007-Cy3

100 μg Cy3 conjugated

$570
PA2007-Dylight488

100 μg Dylight488 conjugated

$570
PA2007-Dylight550

100 μg Dylight550 conjugated

$570
PA2007-Dylight594

100 μg Dylight594 conjugated

$570
PA2007-FITC

100 μg FITC conjugated

$570
PA2007-HRP

100 μg HRP conjugated

$570
PA2007-APC

100 μg APC conjugated

$670
PA2007-PE

100 μg PE conjugated

$670
PA2007-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2007
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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