Anti-TRAM2 Antibody

TRAM2 antibody

Boster Bio Anti-TRAM2 Antibody catalog # PA2011. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2011
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-TRAM2 Antibody

View all TRAM2 Antibodies

SKU/Catalog Number

PA2011

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAM2 Antibody catalog # PA2011. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2011)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2, identical to the related rat sequence, and different from the related human sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2011 is reactive to TRAM2 in Human, Mouse, Rat

Applications

PA2011 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

43.328kDa

Background of TRAM2

TRAM2 (Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane. By genomic sequence analysis, Onuchic et al. (1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al. (2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For TRAM2 (Source: Uniprot.org, NCBI)

Gene Name

TRAM2

Full Name

Translocating chain-associated membrane protein 2

Weight

43.328kDa

Superfamily

TRAM family

Alternative Names

KIAA0057TRAM-like protein; translocating chain-associated membrane protein 2; translocation associated membrane protein 2 TRAM2 translocation associated membrane protein 2 translocating chain-associated membrane protein 2|TRAM-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAM2, check out the TRAM2 Infographic

TRAM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-TRAM2 Antibody

Question

We are currently using anti-TRAM2 antibody PA2011 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

The anti-TRAM2 antibody (PA2011) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-23

Question

Our lab want to know about to test anti-TRAM2 antibody PA2011 on mouse placenta for research purposes, then I may be interested in using anti-TRAM2 antibody PA2011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The products we sell, including anti-TRAM2 antibody PA2011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-29

Question

Does anti-TRAM2 antibody PA2011 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2018-08-07

Answer

According to the expression profile of placenta, TRAM2 is highly expressed in placenta. So, it is likely that anti-TRAM2 antibody PA2011 will work for WB with placenta.

Boster Scientific Support

Answered: 2018-08-07

Question

I was wanting to use your anti-TRAM2 antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?

S. Krishna

Verified customer

Asked: 2014-08-11

Answer

It shows on the product datasheet, PA2011 anti-TRAM2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-08-11

Question

I have a question about product PA2011, anti-TRAM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Mangal

Verified customer

Asked: 2014-07-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2011 anti-TRAM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-07-10

Order DetailsPrice
PA2011

100μg

$370
PA2011-10ug

10μg sample (liquid)

$99
PA2011-Biotin

100 μg Biotin conjugated

$570
PA2011-Cy3

100 μg Cy3 conjugated

$570
PA2011-Dylight488

100 μg Dylight488 conjugated

$570
PA2011-Dylight550

100 μg Dylight550 conjugated

$570
PA2011-Dylight594

100 μg Dylight594 conjugated

$570
PA2011-FITC

100 μg FITC conjugated

$570
PA2011-HRP

100 μg HRP conjugated

$570
PA2011-APC

100 μg APC conjugated

$670
PA2011-PE

100 μg PE conjugated

$670
PA2011-carrier-free

Carrier Free

$370

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PA2011
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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