Anti-Transferrin Purified Azide Free TF Monoclonal Antibody

Product Name

Anti-Transferrin Purified Azide Free TF Monoclonal Antibody

View all Transferrin Antibodies

SKU/Catalog Number

M00094-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (Catalog# M00094-1). Tested in WB, IHC-P, ICC, ELISA, RIA, Functional Assay application(s). This antibody reacts with Human, Pig, Rabbit.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00094-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, azide-free

Clonality

Monoclonal

Clone Number

HTF-14

Isotype

Mouse IgG1

Immunogen

Purified porcine transferrin. The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00094-1 is reactive to TF in Human, Pig, Rabbit

Applications

M00094-1 is guaranteed for ELISA, IHC-P, ICC, WB, Functional Assay, RIA Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

77.064kDa

Background of Transferrin

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Transferrin Purified Azide Free TF Monoclonal Antibody M00094-1

Human transferrin detected by the mouse monoclonal antibody HTF-14.
1. hTransferrin; 5 µg/well (red. con.)
2. hTransferrin; 3 µg/well (red. con.)
3. hTransferrin; 1 µg/well (red. con.)
M Low Range marker (Bio-Rad)
4. hTransferrin; 1 µg/well (non-red. con.)
5. hTransferrin; 3 µg/well (non-red. con.)
6. hTransferrin; 5 µg/well (non-red. con.)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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Figure 2. Immunohistochemistry validation of TF using Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (M00094-1).

Immunohistochemistry staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC

Gene/Protein Information For TF (Source: Uniprot.org, NCBI)

Alternative Names

Beta-1 metal-binding globulin; DKFZp781D0156; EC 3.4.21; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin

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