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Product Info Summary
| SKU: | A03277 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-TRAP ACP5 Antibody
SKU/Catalog Number
A03277
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRAP ACP5 Antibody (Catalog # A03277). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TRAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRAP ACP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03277)
Host
Rabbit
Contents
TRAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRAP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human TRAP. The immunogen is located within the last 50 amino acids of TRAP.
Reactive Species
A03277 is reactive to ACP5 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
36.6 kDa
Background of ACP5
TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03277 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRAP antibody can be used for detection of TRAP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRAP in mouse brain tissue lysate with TRAP antibody at 1 μg/mL.
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Immunofluorescence of TRAP in human liver tissue with TRAP antibody at 20 μg/mL.
Specific Publications For Anti-TRAP ACP5 Antibody (A03277)
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