Click image to see more details
Product Info Summary
| SKU: | M03277-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRAP ACP5 Monoclonal Antibody
SKU/Catalog Number
M03277-1
BM5606 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRAP ACP5 Monoclonal Antibody catalog # M03277-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAP ACP5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03277-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEGH-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase.
Reactive Species
M03277-1 is reactive to ACP5 in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03277-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Positive Control
WB: human A431 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ACP5 using anti-ACP5 antibody (M03277-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP5 antigen affinity purified monoclonal antibody (Catalog # M03277-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACP5 at approximately 37 kDa. The expected band size for ACP5 is at 37 kDa.
Specific Publications For Anti-TRAP ACP5 Monoclonal Antibody (M03277-1)
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6 Customer Q&As for Anti-TRAP ACP5 Monoclonal Antibody
Question
Would anti-TRAP Monoclonal antibody M03277-1 work for IP with spleen?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
According to the expression profile of spleen, ACP5 is highly expressed in spleen. So, it is likely that anti-TRAP Monoclonal antibody M03277-1 will work for IP with spleen.
Boster Scientific Support
Answered: 2020-02-18
Question
I have attached the WB image, lot number and protocol we used for spleen using anti-TRAP Monoclonal antibody M03277-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-TRAP Monoclonal antibody (M03277-1)?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We do provide the blocking peptide for product anti-TRAP Monoclonal antibody (M03277-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-30
Question
My question regarding product M03277-1, anti-TRAP Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Parker
Verified customer
Asked: 2017-09-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03277-1 anti-TRAP Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-15
Question
We are currently using anti-TRAP Monoclonal antibody M03277-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
J. Li
Verified customer
Asked: 2016-12-23
Answer
The anti-TRAP Monoclonal antibody (M03277-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-23
Question
Is this M03277-1 anti-TRAP Monoclonal antibody reactive to the isotypes of ACP5?
O. Singh
Verified customer
Asked: 2015-02-11
Answer
The immunogen of M03277-1 anti-TRAP Monoclonal antibody is A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-11
