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Product Info Summary
| SKU: | M06993 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-TRAP alpha (SSR1) Mouse Monoclonal Antibody [Clone ID: OTI4C7]
SKU/Catalog Number
M06993
Size
100 µl
Description
Boster Bio SSR1 (TRAP alpha) mouse monoclonal antibody, clone OTI4C7 (formerly 4C7). Catalog# M06993. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TRAP alpha (SSR1) Mouse Monoclonal Antibody [Clone ID: OTI4C7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06993)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4C7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human SSR1 (NP_003135) produced in HEK293 cell.
Reactive Species
M06993 is reactive to SSR1 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
32.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06993 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:150
IF 1:50~100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSR1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSR1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either SSR1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSR1 antibody (M06993)
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Anti-SSR1 mouse monoclonal antibody (M06993) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSR1.
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Immunofluorescent staining of HeLa cells using anti-SSR1 mouse monoclonal antibody (M06993).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and SSR1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-SSR1 monoclonal antibody M06993 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-TRAP alpha (SSR1) Mouse Monoclonal Antibody [Clone ID: OTI4C7] (M06993)
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