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Product Info Summary
| SKU: | M00650 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRF2 Monoclonal Antibody
SKU/Catalog Number
M00650
BM5640 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRF2 Monoclonal Antibody catalog # M00650. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00650)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFOB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRF2 Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo.
Reactive Species
M00650 is reactive to TERF2 in Human
Observed Molecular Weight
66 kDa
Calculated molecular weight
59.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00650 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of TRF2 expression in Jurkat cell lysate.
Specific Publications For Anti-TRF2 Monoclonal Antibody (M00650)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-TRF2 Monoclonal Antibody
Question
I have a question about product M00650, anti-TRF2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00650 anti-TRF2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-23
Question
Is a blocking peptide available for product anti-TRF2 Monoclonal antibody (M00650)?
W. Anderson
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-TRF2 Monoclonal antibody (M00650). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-TRF2 Monoclonal antibody M00650 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-17
Question
My team were content with the WB result of your anti-TRF2 Monoclonal antibody. However we have seen positive staining in mammary carcinoma nucleus using this antibody. Is that expected? Could you tell me where is TERF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Based on literature, mammary carcinoma does express TERF2. Generally TERF2 expresses in nucleus. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950
Boster Scientific Support
Answered: 2019-09-11
Question
Our lab used your anti-TRF2 Monoclonal antibody for IF on leukemic t-cell in the past. I am using human, and We want to use the antibody for WB next. you antibody examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
I took a look at the website and datasheets of our anti-TRF2 Monoclonal antibody and it seems that M00650 has been tested on human in both IF and WB. Thus M00650 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-13
Question
Will anti-TRF2 Monoclonal antibody M00650 work on primate IF with adrenal cortex?
S. Carter
Verified customer
Asked: 2019-05-28
Answer
Our lab technicians have not tested anti-TRF2 Monoclonal antibody M00650 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TRF2 Monoclonal antibody M00650 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate adrenal cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-28
Question
Is there a BSA free version of anti-TRF2 Monoclonal antibody M00650 available?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRF2 Monoclonal antibody M00650 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TRF2 Monoclonal antibody M00650. Let me know if you need anything else.
W. Mangal
Verified customer
Asked: 2019-03-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-12
Question
Does M00650 anti-TRF2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
You can see on the product datasheet, M00650 anti-TRF2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-14
Question
We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-TRF2 Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
From what I have seen in literature leukemic t-cell does express TERF2. From what I have seen in Uniprot.org, TERF2 is expressed in frontal cortex, adrenal cortex, mammary carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950
Boster Scientific Support
Answered: 2018-03-29
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TRF2 Monoclonal antibody M00650. Please let me know if you require anything else.
B. Krishna
Verified customer
Asked: 2018-02-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-07
Question
Will anti-TRF2 Monoclonal antibody M00650 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
According to the expression profile of cervix carcinoma erythroleukemia, TERF2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-TRF2 Monoclonal antibody M00650 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-06-21
Question
Is this M00650 anti-TRF2 Monoclonal antibody reactive to the isotypes of TERF2?
Z. Parker
Verified customer
Asked: 2016-05-24
Answer
The immunogen of M00650 anti-TRF2 Monoclonal antibody is A synthesized peptide derived from human TRF2 Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-24
Question
We are currently using anti-TRF2 Monoclonal antibody M00650 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
J. Wu
Verified customer
Asked: 2015-12-24
Answer
The anti-TRF2 Monoclonal antibody (M00650) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-24
Question
you antibody to test anti-TRF2 Monoclonal antibody M00650 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-TRF2 Monoclonal antibody M00650 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Evans
Verified customer
Asked: 2013-09-03
Answer
The products we sell, including anti-TRF2 Monoclonal antibody M00650, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-09-03
Question
I was wanting to use your anti-TRF2 Monoclonal antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
A. Thomas
Verified customer
Asked: 2013-03-29
Answer
As indicated on the product datasheet, M00650 anti-TRF2 Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-29
