Anti-TRF2 Monoclonal Antibody

TRF-2 antibody

Boster Bio Anti-TRF2 Monoclonal Antibody catalog # M00650. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Product Info Summary

SKU: M00650
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-TRF2 Monoclonal Antibody

View all TRF-2 Antibodies

SKU/Catalog Number

M00650

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TRF2 Monoclonal Antibody catalog # M00650. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00650)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFOB-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TRF2 Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00650 is reactive to TERF2 in Human

Applications

M00650 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

59.594kDa

Background of TRF-2

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For TERF2 (Source: Uniprot.org, NCBI)

Gene Name

TERF2

Full Name

Telomeric repeat-binding factor 2

Weight

59.594kDa

Alternative Names

4A794 trf2; 4A794; 4A794.15 trf2; 4A794.15; trf2 4A794; trf2 4A794.15; clone 4A794; clone 4A794.15; Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF 2; TERF2; TRBF 2; TRBF2; TRF 2; TRF2; TRF-2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2 TERF2 TRBF2, TRF2 telomeric repeat binding factor 2 telomeric repeat-binding factor 2|TTAGGG repeat-binding factor 2|telomeric DNA-binding protein|telomeric repeat binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TERF2, check out the TERF2 Infographic

TERF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TERF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-TRF2 Monoclonal Antibody

Question

I have a question about product M00650, anti-TRF2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00650 anti-TRF2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-23

Question

Is a blocking peptide available for product anti-TRF2 Monoclonal antibody (M00650)?

W. Anderson

Verified customer

Asked: 2019-10-17

Answer

We do provide the blocking peptide for product anti-TRF2 Monoclonal antibody (M00650). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-17

Question

I see that the anti-TRF2 Monoclonal antibody M00650 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-17

Question

My team were content with the WB result of your anti-TRF2 Monoclonal antibody. However we have seen positive staining in mammary carcinoma nucleus using this antibody. Is that expected? Could you tell me where is TERF2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Based on literature, mammary carcinoma does express TERF2. Generally TERF2 expresses in nucleus. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950

Boster Scientific Support

Answered: 2019-09-11

Question

Our lab used your anti-TRF2 Monoclonal antibody for IF on leukemic t-cell in the past. I am using human, and We want to use the antibody for WB next. you antibody examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

I took a look at the website and datasheets of our anti-TRF2 Monoclonal antibody and it seems that M00650 has been tested on human in both IF and WB. Thus M00650 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-13

Question

Will anti-TRF2 Monoclonal antibody M00650 work on primate IF with adrenal cortex?

S. Carter

Verified customer

Asked: 2019-05-28

Answer

Our lab technicians have not tested anti-TRF2 Monoclonal antibody M00650 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TRF2 Monoclonal antibody M00650 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate adrenal cortex in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-28

Question

Is there a BSA free version of anti-TRF2 Monoclonal antibody M00650 available?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRF2 Monoclonal antibody M00650 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-10

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TRF2 Monoclonal antibody M00650. Let me know if you need anything else.

W. Mangal

Verified customer

Asked: 2019-03-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-12

Question

Does M00650 anti-TRF2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

You can see on the product datasheet, M00650 anti-TRF2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-14

Question

We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-TRF2 Monoclonal antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

From what I have seen in literature leukemic t-cell does express TERF2. From what I have seen in Uniprot.org, TERF2 is expressed in frontal cortex, adrenal cortex, mammary carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950

Boster Scientific Support

Answered: 2018-03-29

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TRF2 Monoclonal antibody M00650. Please let me know if you require anything else.

B. Krishna

Verified customer

Asked: 2018-02-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-07

Question

Will anti-TRF2 Monoclonal antibody M00650 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2017-06-21

Answer

According to the expression profile of cervix carcinoma erythroleukemia, TERF2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-TRF2 Monoclonal antibody M00650 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2017-06-21

Question

Is this M00650 anti-TRF2 Monoclonal antibody reactive to the isotypes of TERF2?

Z. Parker

Verified customer

Asked: 2016-05-24

Answer

The immunogen of M00650 anti-TRF2 Monoclonal antibody is A synthesized peptide derived from human TRF2 Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-05-24

Question

We are currently using anti-TRF2 Monoclonal antibody M00650 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

J. Wu

Verified customer

Asked: 2015-12-24

Answer

The anti-TRF2 Monoclonal antibody (M00650) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-12-24

Question

you antibody to test anti-TRF2 Monoclonal antibody M00650 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-TRF2 Monoclonal antibody M00650 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Evans

Verified customer

Asked: 2013-09-03

Answer

The products we sell, including anti-TRF2 Monoclonal antibody M00650, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-09-03

Question

I was wanting to use your anti-TRF2 Monoclonal antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

A. Thomas

Verified customer

Asked: 2013-03-29

Answer

As indicated on the product datasheet, M00650 anti-TRF2 Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-03-29

Order DetailsPrice
M00650

100μL

$370
M00650-30ul

30μL sample

$99
M00650-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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