Anti-TRF2/TERF2 Antibody Picoband™

TRF-2 antibody

Boster Bio Anti-TRF2/TERF2 Antibody Picoband™ catalog # A00650-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: A00650-1
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-TRF2/TERF2 Antibody Picoband™

View all TRF-2 Antibodies

SKU/Catalog Number

A00650-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRF2/TERF2 Antibody Picoband™ catalog # A00650-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRF2/TERF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00650-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRF2 recombinant protein (Position: A81-K287). Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00650-1 is reactive to TERF2 in Human, Rat

Applications

A00650-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

59.594kDa

Background of TRF-2

Telomeric repeat-binding factor 2, also known as TERF2, TRF2, or TRBF2, is encoded in humans by the TERF2 gene. This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For TERF2 (Source: Uniprot.org, NCBI)

Gene Name

TERF2

Full Name

Telomeric repeat-binding factor 2

Weight

59.594kDa

Alternative Names

4A794 trf2; 4A794; 4A794.15 trf2; 4A794.15; trf2 4A794; trf2 4A794.15; clone 4A794; clone 4A794.15; Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF 2; TERF2; TRBF 2; TRBF2; TRF 2; TRF2; TRF-2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2 TERF2 TRBF2, TRF2 telomeric repeat binding factor 2 telomeric repeat-binding factor 2|TTAGGG repeat-binding factor 2|telomeric DNA-binding protein|telomeric repeat binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TERF2, check out the TERF2 Infographic

TERF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TERF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-TRF2/TERF2 Antibody Picoband™

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-TRF2/TERF2 antibody A00650-1. Let me know if you need anything else.

D. Jha

Verified customer

Asked: 2020-04-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-29

Question

We have seen staining in human liver. Do you have any suggestions? Is anti-TRF2/TERF2 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

From literature liver does express TERF2. From Uniprot.org, TERF2 is expressed in frontal cortex, adrenal cortex, mammary carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950

Boster Scientific Support

Answered: 2020-03-18

Question

I see that the anti-TRF2/TERF2 antibody A00650-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-12

Question

Is a blocking peptide available for product anti-TRF2/TERF2 antibody (A00650-1)?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

We do provide the blocking peptide for product anti-TRF2/TERF2 antibody (A00650-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-22

Question

Is this A00650-1 anti-TRF2/TERF2 antibody reactive to the isotypes of TERF2?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

The immunogen of A00650-1 anti-TRF2/TERF2 antibody is E.coli-derived human TRF2 recombinant protein (Position: A81-K287). Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-05

Question

I have a question about product A00650-1, anti-TRF2/TERF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00650-1 anti-TRF2/TERF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-15

Question

See below the WB image, lot number and protocol we used for frontal cortex using anti-TRF2/TERF2 antibody A00650-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-19

Question

I was wanting to use your anti-TRF2/TERF2 antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat frontal cortex identification?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

You can see on the product datasheet, A00650-1 anti-TRF2/TERF2 antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-13

Question

Does A00650-1 anti-TRF2/TERF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

It shows on the product datasheet, A00650-1 anti-TRF2/TERF2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-17

Question

We are currently using anti-TRF2/TERF2 antibody A00650-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-20

Answer

The anti-TRF2/TERF2 antibody (A00650-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-20

Question

Will anti-TRF2/TERF2 antibody A00650-1 work for WB with frontal cortex?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

According to the expression profile of frontal cortex, TERF2 is highly expressed in frontal cortex. So, it is likely that anti-TRF2/TERF2 antibody A00650-1 will work for WB with frontal cortex.

Boster Scientific Support

Answered: 2018-06-20

Question

I would like to test anti-TRF2/TERF2 antibody A00650-1 on rat frontal cortex for research purposes, then I may be interested in using anti-TRF2/TERF2 antibody A00650-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Moore

Verified customer

Asked: 2017-06-14

Answer

The products we sell, including anti-TRF2/TERF2 antibody A00650-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-14

Question

Do you have a BSA free version of anti-TRF2/TERF2 antibody A00650-1 available?

G. Banerjee

Verified customer

Asked: 2017-02-13

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TRF2/TERF2 antibody A00650-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-02-13

Question

Our lab want to know about using your anti-TRF2/TERF2 antibody for negative regulation of telomeric d-loop disassembly studies. Has this antibody been tested with western blotting on colo320 whole cell lysates? We would like to see some validation images before ordering.

K. Taylor

Verified customer

Asked: 2016-04-13

Answer

Thank you for your inquiry. This A00650-1 anti-TRF2/TERF2 antibody is tested on colo320 whole cell lysates. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-04-13

Question

We ordered your anti-TRF2/TERF2 antibody for IHC on cervix carcinoma erythroleukemia in a previous project. I am using rat, and We intend to use the antibody for WB next. We are interested in examining cervix carcinoma erythroleukemia as well as adrenal cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

B. Li

Verified customer

Asked: 2014-04-02

Answer

I looked at the website and datasheets of our anti-TRF2/TERF2 antibody and it seems that A00650-1 has been validated on rat in both IHC and WB. Thus A00650-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-04-02

Question

Our team were well pleased with the WB result of your anti-TRF2/TERF2 antibody. However we have observed positive staining in frontal cortex nucleus using this antibody. Is that expected? Could you tell me where is TERF2 supposed to be expressed?

R. Wu

Verified customer

Asked: 2013-01-23

Answer

From literature, frontal cortex does express TERF2. Generally TERF2 expresses in nucleus. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950

Boster Scientific Support

Answered: 2013-01-23

Order DetailsPrice
A00650-1

100μg

$370
A00650-1-10ug

10μg sample (liquid)

$99
A00650-1-Biotin

100 μg Biotin conjugated

$570
A00650-1-Cy3

100 μg Cy3 conjugated

$570
A00650-1-Dylight488

100 μg Dylight488 conjugated

$570
A00650-1-Dylight550

100 μg Dylight550 conjugated

$570
A00650-1-Dylight594

100 μg Dylight594 conjugated

$570
A00650-1-FITC

100 μg FITC conjugated

$570
A00650-1-HRP

100 μg HRP conjugated

$570
A00650-1-APC

100 μg APC conjugated

$670
A00650-1-PE

100 μg PE conjugated

$670
A00650-1-carrier-free

Carrier Free

$370

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