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Product Info Summary
| SKU: | M06479 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]
SKU/Catalog Number
M06479
Size
100 µl
Description
Boster Bio TRIB2 mouse monoclonal antibody, clone OTI8D11 (formerly 8D11). Catalog# M06479. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06479)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8D11
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.
Reactive Species
M06479 is reactive to TRIB2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06479 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000~2000
IHC: 1:50
IF: 1:50
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either TRIB2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIB2 antibody (M06479)
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Immunofluorescent staining of COS7 cells using anti-TRIB2 mouse monoclonal antibody (M06479).
Specific Publications For Anti-TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11] (M06479)
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