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Product Info Summary
| SKU: | A00027-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody
SKU/Catalog Number
A00027-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (Catalog # A00027-3). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027-3)
Host
Rabbit
Contents
JAK2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2. The immunogen is located within the last 50 amino acids of JAK2.
Cross-reactivity
JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.
Reactive Species
A00027-3 is reactive to JAK2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
130.7 kDa
Background of JAK2
JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00027-3 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.
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Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.
Specific Publications For Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (A00027-3)
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