Product Info Summary
| SKU: | A05874-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-UBE2T/Hspc150 Antibody
SKU/Catalog Number
A05874-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UBE2T/Hspc150 Antibody catalog # A05874-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2T/Hspc150 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05874-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human UBE2T. AA range:121-170
Reactive Species
A05874-1 is reactive to UBE2T in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05874-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using UBE2T Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-UBE2T/Hspc150 Antibody (A05874-1)
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Customer Q&As
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4 Customer Q&As for Anti-UBE2T/Hspc150 Antibody
Question
We are currently using anti-UBE2T/Hspc150 antibody A05874-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
M. Johnson
Verified customer
Asked: 2019-09-09
Answer
The anti-UBE2T/Hspc150 antibody (A05874-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Will A05874-1 anti-UBE2T/Hspc150 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
You can see on the product datasheet, A05874-1 anti-UBE2T/Hspc150 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-13
Question
I was wanting to use to test anti-UBE2T/Hspc150 antibody A05874-1 on human adrenal gland for research purposes, then I may be interested in using anti-UBE2T/Hspc150 antibody A05874-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Krishna
Verified customer
Asked: 2016-02-22
Answer
The products we sell, including anti-UBE2T/Hspc150 antibody A05874-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal gland using anti-UBE2T/Hspc150 antibody A05874-1. Let me know if you need anything else.
A. Dhar
Verified customer
Asked: 2013-11-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-18

