Anti-Semaphorin 3B/SEMA3B Antibody Picoband™

Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband™ catalog # A06559-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06559-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Semaphorin 3B/SEMA3B Antibody Picoband™

See all Semaphorin 3B products

SKU/Catalog Number

A06559-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband™ catalog # A06559-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Semaphorin 3B/SEMA3B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06559-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A06559-1 is reactive to SEMA3B in Human, Mouse, Rat

Applications

A06559-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SEMA3B (Source: Uniprot.Org, NCBI)

Uniprot ID

Q13214

Gene ID

7869

Gene Name

SEMA3B

Full Name

Semaphorin-3B

Weight

83.122kDa

Superfamily

semaphorin family

Alternative Names

(semaphorin) 3B; LUCA-1; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema V; SemA; SEMA3B; SEMA5; SEMA5FLJ34863; SEMAA; Semaphorin 3B; semaphorin A; semaphorin V; semaphorin-3B; semaphorin-V; SEMAV SEMA3B LUCA-1, SEMA5, SEMAA, SemA, semaV semaphorin 3B semaphorin-3B|sema A(V)|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B|semaphorin A|semaphorin-V

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SEMA3B, check out the SEMA3B Infographic

SEMA3B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SEMA3B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-Semaphorin 3B/SEMA3B Antibody Picoband™

Question

See below the WB image, lot number and protocol we used for brain muscle using anti-Semaphorin 3B/SEMA3B antibody A06559-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-17

Question

Will A06559-1 anti-Semaphorin 3B/SEMA3B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-14

Answer

You can see on the product datasheet, A06559-1 anti-Semaphorin 3B/SEMA3B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-14

Question

We are currently using anti-Semaphorin 3B/SEMA3B antibody A06559-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

The anti-Semaphorin 3B/SEMA3B antibody (A06559-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-28

Question

Do you have a BSA free version of anti-Semaphorin 3B/SEMA3B antibody A06559-1 available?

Verified Customer

Verified customer

Asked: 2017-08-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Semaphorin 3B/SEMA3B antibody A06559-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-17

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A06559-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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