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UBB antibody (M02848-3) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunofluorescent analysis of Raji cells, using Ubiquitin Antibody .
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Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
UBB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
UBB antibody was positively detected by WB in:
human MCF-7 whole cell, human U251 whole cell, human A549 whole cell, human Caco-2 whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
UBB antibody was positively detected by IHC in:
Human breast cancer tissue, Human esophageal carcinoma tissue, human colon tissue, Human stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
UBB antibody was positively detected by ICC/IF in:
Raji cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
View all UBB Antibodies
View all UBB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DIG-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ubiquitin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
10 kDa
Calculated Molecular Weight
25.8 kDa
Cite This Product
Anti-Ubiquitin UBB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02848-3)
Protocols
Western Blot protocol for UBB antibody
Immunohistochemistry (IHC) protocol for UBB antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for UBB antibody
Publications
Application:
Species:
Discovery of SKP2-Recruiting PROTACs for Target Protein Degradation
Published: 2026-02-04
Journal: Advanced Science
Application: WB
Impact Factor: 14.10
Species: Human
PMID: 41637562
Forsythiaside A ameliorates acetaminophen-induced liver injury by suppressing ferroptosis through activating USP11/SIRT3-mediated mitophagy
Published: 2025-12-01
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Human
PMID: 41338315
M6A-mediated uc003pes.1 stability regulates proliferation and migration of human coronary artery smooth muscle cells via scaffolding STAT1 and USP10
Published: 2025-07-15
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 40669772
Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation
Published: 2022-01-28
Journal: Cell and Bioscience
Application: WB
Impact Factor: 9.58
Species: Rat
PMID: 35090552
Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana
Published: 2021-03-08
Journal: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Application: WB
Impact Factor: 4.81
Species: B. bassiana
PMID: 33686455
Pleiotropic effects of Ubi4, a polyubiquitin precursor required for ubiquitin accumulation, conidiation and pathogenicity of a fungal insect pathogen
Published: 2020-02-13
Journal: ENVIRONMENTAL MICROBIOLOGY
Application: WB
Impact Factor: 4.93
Species: B. Bassiana
PMID: 32056334
The degradation of kinesin-like calmodulin binding protein of D. salina (DsKCBP) is mediated by the ubiquitin–proteasome system
Published: 2012-12-28
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB
Impact Factor: 2.93
Species: Dunaliella salina
PMID: 23271117
Review
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Product Promise
We promise Anti-Ubiquitin UBB Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02848-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026