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Product Info Summary
| SKU: | A10658 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-V-ATPase D1 ATP6V0D1 Antibody
SKU/Catalog Number
A10658
Size
100μl
Form
Liquid
Description
Boster Bio Anti-V-ATPase D1 ATP6V0D1 Antibody catalog # A10658. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-V-ATPase D1 ATP6V0D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10658)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human V-ATPase D1. AA range:221-270
Reactive Species
A10658 is reactive to ATP6V0D1 in Human, Mouse, Rat
Calculated molecular weight
40.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10658 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from NIH-3T3, K562 cells, using V-ATPase D1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from HeLa cells, using V-ATPase D1 antibody.
Specific Publications For Anti-V-ATPase D1 ATP6V0D1 Antibody (A10658)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-V-ATPase D1 ATP6V0D1 Antibody
Question
Do you have a BSA free version of anti-V-ATPase D1 antibody A10658 available?
K. Jones
Verified customer
Asked: 2020-04-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-V-ATPase D1 antibody A10658 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this A10658 anti-V-ATPase D1 antibody reactive to the isotypes of ATP6V0D1?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of A10658 anti-V-ATPase D1 antibody is Synthesized peptide derived from the Internal region of human V-ATPase D1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
We are currently using anti-V-ATPase D1 antibody A10658 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The anti-V-ATPase D1 antibody (A10658) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-V-ATPase D1 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
E. Patel
Verified customer
Asked: 2018-04-30
Answer
You can see on the product datasheet, A10658 anti-V-ATPase D1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-30
Question
Would anti-V-ATPase D1 antibody A10658 work on pig WB with osteoclastoma?
S. Jackson
Verified customer
Asked: 2015-02-26
Answer
Our lab technicians have not validated anti-V-ATPase D1 antibody A10658 on pig. You can run a BLAST between pig and the immunogen sequence of anti-V-ATPase D1 antibody A10658 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig osteoclastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-26
