Click image to see more details
Product Info Summary
| SKU: | PA2189 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®
SKU/Catalog Number
PA2189
BA3245 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Monocarboxylate transporter 5 SLC16A4 Antibody catalog # PA2189. Tested in ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2189)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4, different from the related rat and mouse sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2189 is reactive to SLC16A4 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.0 kDa
Background of SLC16A4
Monocarboxylate transporter 5, also called Solute carrier family 16 member 4 or MCT4, is a protein that in humans is encodes by the SLC16A4 gene. This gene is mapped to 1p13.3. It acts as the proton-linked monocarboxylate transporter. This gene catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2189 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human A549 whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue
IHC: human kidney tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC16A4 antigen affinity purified polyclonal antibody (PA2189) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC16A4 at approximately 54 kDa. The expected band size for SLC16A4 is at 54 kDa.
Click image to see more details
IHC analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189).
SLC16A4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC16A4 Antibody (PA2189) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IF analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189).
SLC16A4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC16A4 Antibody (PA2189) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband® (PA2189)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®
Question
I was wanting to use your anti-SLC16A4 antibody for IHC for mouse palpebral conjunctiva on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse palpebral conjunctiva identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
It shows on the product datasheet, PA2189 anti-SLC16A4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse palpebral conjunctiva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
Is this PA2189 anti-SLC16A4 antibody reactive to the isotypes of SLC16A4?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The immunogen of PA2189 anti-SLC16A4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4(134-148aa VVTTKYFKKRLALST), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-09
Question
Can you help my question with product PA2189, anti-SLC16A4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2189 anti-SLC16A4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
I see that the anti-SLC16A4 antibody PA2189 works with IHC, what is the protocol used to produce the result images on the product page?
P. Jackson
Verified customer
Asked: 2018-08-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-31
Question
My lab would like to test anti-SLC16A4 antibody PA2189 on mouse palpebral conjunctiva for research purposes, then I may be interested in using anti-SLC16A4 antibody PA2189 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
The products we sell, including anti-SLC16A4 antibody PA2189, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-22
Question
We are currently using anti-SLC16A4 antibody PA2189 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
L. Walker
Verified customer
Asked: 2016-01-26
Answer
The anti-SLC16A4 antibody (PA2189) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-26
Question
Will anti-SLC16A4 antibody PA2189 work on monkey WB with placenta substantia nigra?
A. Zhang
Verified customer
Asked: 2015-02-09
Answer
Our lab technicians have not validated anti-SLC16A4 antibody PA2189 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SLC16A4 antibody PA2189 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta substantia nigra in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-09
