|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-VEGFD Antibody|
|Description||Rabbit IgG polyclonal antibody for Vascular endothelial growth factor D(FIGF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-VEGFD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1332)|
|Replacement Item||This antibody may replace the following items: sc-13085|sc-25784|sc-27130|sc-27131|sc-373866|sc-7602|sc-7603 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human VEGFD(101-115aa VIDEEWQRTQCSPRE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Vascular endothelial growth factor D|
|Molecular Weight||40444 MW|
|Protein Function||Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. .|
|Tissue Specificity||Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.|
|Sequence Similarities||Belongs to the PDGF/VEGF growth factor family.|
|Alternative Names||Vascular endothelial growth factor D;VEGF-D;c-Fos-induced growth factor;FIGF;FIGF;VEGFD;|
|Research Areas|||cardiovascular|angiogenesis|growth factors|vegf| signal transduction|growth factors/hormones| cancer|invasion/microenvironment|angiogenic growth factors||
Background for Vascular endothelial growth factor D
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-VEGFD Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-VEGFD Antibody Images
Click the images to enlarge.
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: 6T-CEM Cell Lysate
Lane 4: HT1080 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,