Product Info Summary
SKU: | M03951 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-VPS26 VPS26A Monoclonal Antibody
SKU/Catalog Number
M03951
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VPS26 VPS26A Monoclonal Antibody catalog # M03951. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS26 VPS26A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03951)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEEE-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VPS26
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M03951 is reactive to VPS26A in Human, Mouse, Rat
Applications
M03951 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
38.17kDa
Background of VPS26A
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of VPS26 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For VPS26A (Source: Uniprot.org, NCBI)
Gene Name
VPS26A
Full Name
Vacuolar protein sorting-associated protein 26A
Weight
38.17kDa
Superfamily
VPS26 family
Alternative Names
FLJ12930; HB58; Hbeta58; hVPS26; PEP8A; vacuolar protein sorting 26 (yeast homolog); vacuolar protein sorting 26 homolog A (S. pombe); vacuolar protein sorting 26 homolog A (yeast); vacuolar protein sorting-associated protein 26A; Vesicle protein sorting 26A; VPS26vacuolar protein sorting 26 (yeast) VPS26A HB58, Hbeta58, PEP8A, VPS26 VPS26, retromer complex component A vacuolar protein sorting-associated protein 26A|VPS26 retromer complex comonent A|vacuolar protein sorting 26 homolog A|vesicle protein sorting 26A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS26A, check out the VPS26A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS26A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS26 VPS26A Monoclonal Antibody (M03951)
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No publications found for M03951
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-VPS26 VPS26A Monoclonal Antibody
Question
I see that the anti-VPS26 Monoclonal antibody M03951 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-30
Question
Is a blocking peptide available for product anti-VPS26 Monoclonal antibody (M03951)?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
We do provide the blocking peptide for product anti-VPS26 Monoclonal antibody (M03951). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-23
Question
We are currently using anti-VPS26 Monoclonal antibody M03951 for mouse tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
The anti-VPS26 Monoclonal antibody (M03951) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-01
Question
I have a question about product M03951, anti-VPS26 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Patel
Verified customer
Asked: 2016-08-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03951 anti-VPS26 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-04
Question
Does anti-VPS26 Monoclonal antibody M03951 work for WB with brain embryonic testis carcinoma?
L. Anderson
Verified customer
Asked: 2014-03-24
Answer
According to the expression profile of brain embryonic testis carcinoma, VPS26A is highly expressed in brain embryonic testis carcinoma. So, it is likely that anti-VPS26 Monoclonal antibody M03951 will work for WB with brain embryonic testis carcinoma.
Boster Scientific Support
Answered: 2014-03-24