Anti-Annexin A10/ANXA10 Antibody Picoband®

Product Name

Anti-Annexin A10/ANXA10 Antibody Picoband®

View all ANXA10 Antibodies

SKU/Catalog Number

PA2077
BA3034 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

ANXA10 (annexin A10) is typically studied as an annexin-family phospholipid-binding protein associated with membrane organization and epithelial differentiation programs (putative here due to page access failure), and has context-dependent use in tumor/epithelial marker panels (putative). Assay context: validated for IHC and Western blot with Human/Rat reactivity; membrane/traffic interpretation is often compared with small-GTPase trafficking regulators such as ARF6 (putative) and growth factor signaling contexts such as EGF (putative), with histology interpretation optionally supported by pathology review (method-context).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin A10/ANXA10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2077)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin A10, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2077 is reactive to ANXA10 in Human, Rat

Observed Molecular Weight

50 kDa

Calculated molecular weight

37.3 kDa

Background of ANXA10

ANXA10 (Annexin A10) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. By FISH, Morgan et al. (1999) mapped the ANXA10 gene to chromosome 4q33. Morgan et al. (1999) suggested that the ablation or depletion of principal calcium-binding sites in annexins A9 and A10, respectively, may dispose them to function in distinct tissue environments where calcium sensitivity is not a major functional determinant.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2077 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: A549 Cell, A549 Cell,
IHC: Human Lung Cancer Tissue

Validation Images & Assay Conditions

See full target information ANXA10

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