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Product Info Summary
| SKU: | M10150 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-WDR4 Mouse Monoclonal Antibody [Clone ID: OTI4G1]
SKU/Catalog Number
M10150
Size
100 µl
Description
Boster Bio WDR4 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1). Catalog# M10150. Tested in FC, IF, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-WDR4 Mouse Monoclonal Antibody [Clone ID: OTI4G1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10150)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4G1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human WDR4 (NP_387510) produced in HEK293T cell.
Reactive Species
M10150 is reactive to WDR4 in Human
Observed Molecular Weight
45 kDa
Calculated molecular weight
45.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10150 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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Western blot analysis of WDR4 using anti-WDR4 antibody (M10150).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-WDR4 antigen affinity purified monoclonal antibody (Catalog # M10150) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for WDR4 at approximately 45 kDa. The expected band size for WDR4 is at 45 kDa.
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HEK293T cells transfected with either WDR4 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-WDR4 antibody (M10150)
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Anti-WDR4 mouse monoclonal antibody (M10150) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY WDR4.
Specific Publications For Anti-WDR4 Mouse Monoclonal Antibody [Clone ID: OTI4G1] (M10150)
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