Human COMT ELISA Kit PicoKine™

Human COMT ELISA Kit PicoKine™ (96 Tests). Quantitate Human COMT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1406
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human COMT ELISA Kit PicoKine™

See all COMT products

SKU/Catalog Number

EK1406

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human COMT ELISA Kit PicoKine™ (96 Tests). Quantitate Human COMT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human COMT ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1406)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G52-P271

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1406 is reactive to COMT in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1406 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1406-CAP Anti-Human COMT Pre-coated 96-well strip microplate 1
EK1406-ST Human COMT Standard 2 vials, 10 ng/tube
EK1406-DA Human COMT Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0260.0870.1340.2750.4920.9041.4922.223

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect COMT, Dilution ratio of 1:1, concentration in serum is 121-214pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human COMT ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7124592365220946
Standard deviation4.9716.1761.848.4316.579.46
CV (%)7%6.6%6.7%7.9%7.5%8.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of COMT in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 171726762683.935.7%
Sample 224524527927025915.085.8%
Sample 392310611051966100057.895.7%
*number of samples for each test n=16.

Gene/Protein Information For COMT (Source: Uniprot.Org, NCBI)

Uniprot ID

P21964

Gene ID

1312

Gene Name

COMT

Full Name

Catechol O-methyltransferase

Weight

30.037kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

catechol O-methyltransferase; catechol-O-methyltransferase; COMT; EC 2.1.1.6 COMT HEL-S-98n catechol-O-methyltransferase catechol O-methyltransferase|epididymis secretory sperm binding protein Li 98n|testicular tissue protein Li 42

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COMT, check out the COMT Infographic

COMT infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COMT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1406

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Find answers in Q&As, reviews.

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10 Customer Q&As for Human COMT ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I measured COMT level in cell lysates.

Verified Customer

Verified customer

Asked: 2020-01-11

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-01-11

Question

What buffer is recommended with cell lysate using the EK1406 kit?

Verified customer

Asked: 2019-12-23

Answer

The recommended buffer with cell lysate using the Human COMT ELISA Kit PicoKine EK1406 is IP Lysis Buffer AR0107.

Boster Scientific Support

Answered: 2019-12-27

Question

Was a baculovirus expression system used for the manufacturing process of EK1406?

Verified customer

Asked: 2019-12-01

Answer

The Human COMT ELISA Kit PicoKine™ (EK1406) does not use baculovirus expression system. However our ELISA kits that has the expression systems of sf9 and sf21 use baculovirrus expression system.

Boster Scientific Support

Answered: 2019-12-03

Question

Has EK1406 kit been tested on human feces samples?

Verified customer

Asked: 2019-08-22

Answer

Our lab has not tested the Human COMT ELISA Kit PicoKine EK1406 on human feces samples before. There is no guarantee that the ELISA kit will work for the samples since we do not have much experience in testing feces samples. It is suggested to make sure that the samples are liquid without any solid impurities prior to use if doing a pilot test.

Boster Scientific Support

Answered: 2019-08-23

Question

Q: Can COMT ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-04-11

Question

Q: if the enzyme conjugated COMT antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the COMT antigen?

Verified Customer

Verified customer

Asked: 2018-09-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-09-23

Question

Q: can I use citrate plasma as samples in Human COMT Picokine® ELISA Kit (Catalog # EK1406)?

W. Surve

Verified customer

Asked: 2018-02-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of COMT causing degradation of its protein structure. COMT may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the COMT ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-02-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Phillips

Verified customer

Asked: 2018-02-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-02-11

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

J. Zhang

Verified customer

Asked: 2017-06-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-06-10

Question

Q: how to thaw whole blood sample for COMT ELISA after freezing?

Verified Customer

Verified customer

Asked: 2015-11-02

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test COMT for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2015-11-02

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