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Product Info Summary
| SKU: | A06521 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-WHIP WRNIP1 Antibody
SKU/Catalog Number
A06521
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-WHIP WRNIP1 Antibody (Catalog # A06521). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-WHIP WRNIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06521)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Reactive Species
A06521 is reactive to WRNIP1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
72.1 kDa
Background of WRNIP1
Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06521 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:40,000
IHC: User optimized
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Validation Images & Assay Conditions
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Western blot analysis is shown using Boster's Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Western blot analysis is shown using Boster's anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30µg of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other systems will yield similar results.
Specific Publications For Anti-WHIP WRNIP1 Antibody (A06521)
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1 Customer Q&As for Anti-WHIP WRNIP1 Antibody
Question
We are currently using anti-WHIP antibody A06521 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
J. Dhar
Verified customer
Asked: 2017-04-11
Answer
The anti-WHIP antibody (A06521) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-11
