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Product Info Summary
| SKU: | A03360 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ALKBH5 Antibody Picoband®
SKU/Catalog Number
A03360
Size
100 μg/vial
Form
Lyophilized
Description
ALKBH5 is an mRNA N6-methyladenosine (m6A) demethylase that influences RNA metabolism (e.g., splicing/processing, stability, export) via reversible m6A marking, making it a common handle for epitranscriptomic regulation studies. Assay context: human-reactive antibody tested in ELISA, IF/ICC, and WB. Frequently compared with RNA-silencing complex components such as AGO2 and AGO3 when linking RNA modification state to post-transcriptional control phenotypes (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALKBH5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03360)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03360 is reactive to ALKBH5 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
44.3 kDa
Background of ALKBH5
RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N (6)-methyladenosine (m (6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m (6)A from mRNAs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03360 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human 293T whole cell, human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human U87 whole cell
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog # A03360) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52 kDa. The expected band size for ALKBH5 is at 44 kDa.
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IF analysis of ALKBH5 using anti-ALKBH5 antibody (A03360) and anti-Tubulin Alpha antibody (M03989-3).
ALKBH5 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALKBH5 Antibody (A03360) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-ALKBH5 Antibody Picoband® (A03360)
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