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Product Info Summary
| SKU: | A00234-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-XBP-1 Antibody
SKU/Catalog Number
A00234-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-XBP-1 Antibody (Catalog # A00234-1). Tested in ELISA, WB, ICC, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
XBP-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-XBP-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00234-1)
Host
Rabbit
Contents
XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-XBP-1 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
Reactive Species
A00234-1 is reactive to XBP1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.7 kDa
Background of XBP1
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00234-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25-2 μg/mL; IF: 20 μg/mL; ICC: 10 μg/mL; IHC: 2-5 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunofluorescence Validation of XBP-1 in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with A00234-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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XBP-1 KO Validation in 293T Cells
Loading: 10 μg of lysate
Antibodies: XBP-1 A00234-1, 4 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation with Recombinant Protein
Loading: 100 ng of human XBP-1 recombinant protein per lane.
Antibodies: XBP-1 A00234-1 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Observed at around 23kD.
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Western Blot Validation of XBP-1 in HepG2 Cells
Loading: 15 μg of HepG2 cell lysate
Antibodies: XBP-1 A00234-1, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane1: 1 μg/mL
Lane2: 2 μg/mL
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Western Blot Validation in Mouse Pancreas Tissue
Loading: 15 μg of lysates per lane.
Antibodies: XBP-1, A00234-1 (4 μg /mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Liver Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: XBP-1 A00234-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
(1) the absence and
(2) the presence of blocking peptide
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Immunofluorescence Validation of XBP-1 in Human Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with A00234-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunofluorescence Validation of XBP-1 in Human Liver Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with A00234-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunofluorescence Validation of XBP-1 in Mouse Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with A00234-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry Validation of XBP-1 in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP-1 antibody (A00234-1) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of XBP-1 in Mouse Spleen Tissue
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP-1 antibody (A00234-1) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of XBP-1 in Rat Spleen Tissue
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP-1 antibody (A00234-1) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-XBP-1 Antibody (A00234-1)
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