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- Table of Contents
XBP1 antibody (A00234-1) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of XBP-1 in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with A00234-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with A00234-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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Anti-XBP-1 Antibody
XBP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.25-2 μg/mL; IF: 20 μg/mL; ICC: 10 μg/mL; IHC: 2-5 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human
mouse
and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-XBP-1 Antibody
View all XBP1 Antibodies
View all XBP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
XBP-1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
XBP-1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-XBP-1 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
28.7 kDa
Background of XBP1
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Cite This Product
Anti-XBP-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00234-1)
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Product Promise
We promise Anti-XBP-1 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00234-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026