Rabbit IgG polyclonal antibody for DNA repair protein XRCC1(XRCC1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Anti-XRCC1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
EPH Receptor A2 (EPHA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPHA2 is a protein which binds ephrin-A ligands. EPH and EPH-related receptors are associated with mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily normally have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA2 gene mutations are the cause of certain genetically-related cataract disorders.
|Product Name||Anti-XRCC1 Antibody
See all XRCC1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for XRCC1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. XRCC1 information: Molecular Weight: 69477 MW; Subcellular Localization: Nucleus . Accumulates at sites of DNA damage.|
|Cite This Product||Anti-XRCC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1639)|
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|Anti-XRCC1 Antibody may replace the following items: sc 11429|sc 5902|sc 5903|sc 56254.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1(15-34aa QDSTHCAENLLKADTYRKWR), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For XRCC1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7515|
|Species Of This Entry||Human|
|Protein Name||DNA repair protein XRCC1|
|Alternative Names||XRCC1|DNA repair protein XRCC1; RCC; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Ubiquitination; Oxidation; Ribosylation; Acetylation; Deamination; Sumoylation; Demethylation; Dephosphorylation; Reduction; Deacetylation|
|Subcellular Localization||Nucleus. Moves from the nucleoli to the global nuclear chromatin upon DNA damage.|
|Gene Location||19q13.31, on Chromasome 19, gene sequence: NC_000019.10 (43543311..43575527, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For XRCC1 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.|
|Tissue Specificity||Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).|
|Interacting Proteins||APLF; APTX; CHEK2; MAX; PARP1; PNKP; POLB; Polb; POLI|
|Research Areas||Apoptosis; Base Excision Repair; Cancer; DNA Repair; Homologous Recombination; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Neoplasm Of Lung; Malignant Neoplasm Of Breast; Lung Neoplasms; Mammary Neoplasms; Malignant Squamous Cell Neoplasm; Carcinogenesis; Adenocarcinoma; Colorectal Cancer; Non-small Cell Lung Carcinoma; Colorectal Neoplasms; Xeroderma; Xeroderma Pigmentosum; 5,10-methylenetetrahydrofolate Reductase Deficiency; Cytogenetic Abnormality; Leukemia; Stomach Neoplasms; Cholangiocarcinoma|
|Related Pathways||Dna Repair; Cell Cycle; Pathogenesis; Base-excision Repair; Dna Replication; Cell Death; Mismatch Repair; Hypersensitivity; Localization; Methylation; Double-strand Break Repair; Aging; Cell Proliferation; Single Strand Break Repair; Drug Resistance; Metaphase; S Phase; Cell Cycle Checkpoint; Dna Methylation; Cell Growth|
|Background||XRCC1(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.|
|Scientific References||PMID: 2247054 by Thompson L.H., et al. Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange.|
PMID: 11163244 by Whitehouse C.J., et al. XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair.
Our Boster Quality Guarantee for Anti-XRCC1 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: 293T Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: HELA Cell Lysate
IHC(P): Human Mammary Cancer Tissue
ICC: A549 Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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