Anti-ZBTB8 Antibody

Product Name

Anti-ZBTB8 Antibody

View all ZBTB8A Antibodies

SKU/Catalog Number

A12536

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZBTB8 Antibody (Catalog # A12536). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ZBTB8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZBTB8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12536)

Host

Rabbit

Contents

ZBTB8 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZBTB8 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB8. The immunogen is located within the last 50 amino acids of ZBTB8.

Cross-reactivity

At least two isoforms of ZBTB8 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.

Reactive Species

A12536 is reactive to ZBTB8A in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.1 kDa

Background of ZBTB8A

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB8, also known as BOZ-F1, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB8, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB8 may act as a transcriptional repressor or be involved in tumorigenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12536 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZBTB8 antibody can be used for detection of ZBTB8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ZBTB8A

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