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Product Info Summary
| SKU: | A12548 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB9 Antibody
SKU/Catalog Number
A12548
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB9 Antibody (Catalog # A12548). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ZBTB9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12548)
Host
Rabbit
Contents
ZBTB9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB9 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ZBTB9. The immunogen is located within the first 50 amino acids of ZBTB9.
Cross-reactivity
This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A12548 is reactive to ZBTB9 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.6 kDa
Background of ZBTB9
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB9, also known as ZNF919, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB9, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB9 may act as a transcriptional repressor or be involved in tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12548 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB9 antibody can be used for detection of ZBTB9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB9 in mouse heart tissue lysate with ZBTB9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 2.5 μg/mL.
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Immunofluorescence of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 20 μg/mL.
Specific Publications For Anti-ZBTB9 Antibody (A12548)
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