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CYP7A1 antibody (AZF1QU76) performs well in IF, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of CYP7A1 using anti-CYP7A1 antibody (AZF1QU76).
CYP7A1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CYP7A1 Antibody (AZF1QU76) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CYP7A1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CYP7A1 Antibody (AZF1QU76) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish CYP7A1 Antibody Picoband®
CYP7A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, WB |
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunofluorescence
5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish CYP7A1 Antibody Picoband®
View all CYP7A1 Antibodies
View all CYP7A1 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish CYP7A1 recombinant protein (Position: H102-L512).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
59 kDa
Background of CYP7A1
Predicted to enable steroid hydroxylase activity. Predicted to be involved in bile acid biosynthetic process and cholesterol homeostasis. Predicted to act upstream of or within cellular response to glucose stimulus and cholesterol catabolic process. Predicted to be located in endoplasmic reticulum membrane. Is expressed in basal plate midbrain region; central nervous system; liver; otic vesicle; and retinal ganglion cell layer. Orthologous to human CYP7A1 (cytochrome P450 family 7 subfamily A member 1).
Cite This Product
Anti-Zebrafish CYP7A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1QU76)
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Product Promise
We promise Anti-Zebrafish CYP7A1 Antibody Picoband® performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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AZF1QU76
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Special Price
$99.00
Regular Price
$470.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026