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EIF4A2 antibody (AZF1R166) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166).
EIF4A2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
EIF4A2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot or IHC for EIF4A2? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Zebrafish EIF4A2 Antibody Picoband®
EIF4A2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
EIF4A2 antibody was positively detected by IF in:
zebrafish embryo tissue
EIF4A2 antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EIF4A2 antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish colon tissue, zebrafish kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Zebrafish
Tested
Tested
Tested
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Human
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Zebrafish EIF4A2 Antibody Picoband®
View all EIF4A2 Antibodies
View all EIF4A2 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish EIF4A2 recombinant protein (Position: M1-Q119).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
46 kDa
Background of EIF4A2
Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.
Cite This Product
Anti-Zebrafish EIF4A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1R166)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Human
92%
High Homology
Mouse
92%
High Homology
Rat
92%
High Homology
Rabbit
92%
High Homology
Dog
92%
High Homology
Monkey
92%
High Homology
Drosophila
66%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunofluorescence (IF) protocol for EIF4A2 antibody
Western Blot protocol for EIF4A2 antibody
Immunohistochemistry (IHC) protocol for EIF4A2 antibody
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Product Promise
We promise Anti-Zebrafish EIF4A2 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZF1R166
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026