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Product Info Summary
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Anti-ZP3 Antibody Picoband™
Boster Bio Anti-ZP3 Antibody Picoband™ catalog # A03543-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03543-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human ZP3 (LRLMEENWNAEKRSPTFHLGDAAHLQAEIHT).
No cross reactivity with other proteins.
A03543-1 is reactive to ZP3 in Human
A03543-1 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZP3 using anti-ZP3 antibody (A03543-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U2OS whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP3 antigen affinity purified polyclonal antibody (Catalog # A03543-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP3 at approximately 47KD. The expected band size for ZP3 is at 47KD.
Gene/Protein Information For ZP3 (Source: Uniprot.Org, NCBI)
Zona pellucida sperm-binding protein 3
ZP domain family
zona pellucida glycoprotein 3 (sperm receptor) ZP3 OOMD3A, ZP3B, ZPC, Zp-3, ZP3 zona pellucida glycoprotein 3 zona pellucida sperm-binding protein 3|ZP3A/ZP3B|sperm receptor|zona pellucida glycoprotein 3B|zona pellucida glycoprotein ZP3|zona pellucida protein C*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ZP3, check out the ZP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ZP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A03543-1
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