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Facts about ATP-dependent translocase ABCB1.
Human | |
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Gene Name: | ABCB1 |
Uniprot: | P08183 |
Entrez: | 5243 |
Belongs to: |
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ABC transporter superfamily |
ABC20; ABCB1; ABCB1B; ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CD243 antigen; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; EC 3.6.3; EC 3.6.3.44; GP170; IBD13; MDR1; MDR1MGC163296; multidrug resistance protein 1; P-glycoprotein 1; P-gp; PGY1; PGY1P-GP
Mass (kDA):
141.479 kDA
Human | |
---|---|
Location: | 7q21.12 |
Sequence: | 7; NC_000007.14 (87503017..87713323, complement) |
Expressed in liver, kidney, small intestine and brain.
Cell membrane; Multi-pass membrane protein. Apical cell membrane.
One of the best ways to maximize the potential of your experiments is to optimize your biological markers using Boster Bio. These guides can answer several questions and guide your experiment optimization. You can use these guides to improve your results and minimize the chances of experiment errors. No matter how well-designed your experiments are, you will experience a number of troubleshooting issues. You can minimize these issues by using proper controls and troubleshooting guides.
If you're looking for a high-quality antibody for Western Blotting, Boster Bio is a good choice. This company provides quality primary antibodies and ELISA kits. Its laboratory produces its products under rigorous quality standards to ensure a consistent and high-quality product. Whether you're using Boster antibodies for ELISA or WB, they are guaranteed to work as described.
To ensure reproducibility of antibody performance, collaboration between user, vendor, and publisher is essential. The user must conduct well-designed experiments to validate the antibody's performance. The vendor should provide high-quality antibodies with comprehensive disclosure of their methods. The publisher can help by formulating and enforcing validation guidelines for reporting results. Finally, it's essential for all of these stakeholders to work together to improve antibody reproducibility and quality control.
A positive control is a cell line or lysate that expresses the target protein. If the control lane shows a positive result, it means that the antibody successfully detected the target protein. Conversely, a negative result indicates that the antibody's selectivity was compromised. It's important to use positive controls to validate antibodies on Western blotting experiments because they help to identify potential sources of error before they have a significant impact on results.
Moreover, secondary antibodies should be validated against all species present in the immunoassay. If the antibodies are not specific enough to detect the target protein, it is important to perform separate Western blottings with different secondary antibodies. The presence of overlapping bands may affect data analysis and interpretation. To avoid this, PTM directed antibodies should have high specificity. This ensures a high-quality antibody.
Immunoassays vary in the specificity and selectivity of primary antibodies. Generally, KO validation is considered the gold standard for Western blotting and is often used by antibody vendors during development and batch testing. However, as with many laboratory procedures, an antibody's performance and reproducibility depends on its context. Therefore, it's vital to choose an antibody with assay-specific validation.
Using a chemiluminescent imaging system, Bio-Rad's Odyssey(r) CLx imaging system was used to visualize primary antibody binding. It was also used to identify the primary antibody by detecting chemiluminescent signals. Finally, images were prepared by using Adobe Photoshop Elements 5.0 and 13.0. So, if you're looking for an antibody that's both powerful and high-quality, consider Boster Bio.
The ABCB1 is an anti-glycoprotein antibody from Boster Bio. This antibody has been tested for specificity and affinity in ELISA and flow cytometry. It reacts with Human, Mouse and Rat. Boster Bio's anti-ABCB1 antibody is a part of the Picoband(tm) catalog. Boster Bio offers quality lysis buffers for use in ELISA, flow cytometry, and other experiments.
ELISA for the ABCB1 marker measures the protein ABCB1. It is a member of the xenobiotic transporting ATPase class. Its predicted amino acid sequence is 1280 amino acids long, and its mass is 141.5 kDa. It is a membrane-subcellular protein with glycosylation sites and pathologic involvement in inflammation. Its relative fluorescence intensity was reduced in Aq-MDR cells, when transferred to Aq-MDR cells. The transfer of ABCB1 has two major functions: to protect KB cells from cytotoxicity and to pump out chemotherapeutic drugs.
The ELISA for the ABCB1 marker requires optimized plate-coating conditions. The assay microplate must contain a minimum protein binding capacity of 400 ng/cm2 to achieve good sensitivity. The CV value should be low so that the results obtained are consistent. The plate color also depends on the type of signal. Colorimetric signals need to be plated on transparent polystyrene flat bottom plates, whereas fluorescent signals need black or white opaque plates.
ELISA for the ABCB1 marker uses an enzymatic reaction to detect the protein. The enzyme-substrate complex then oxidizes a luminol and emits light. This light is generated only when the enzyme-substrate complex reacts with the substrate. The reaction also ends when the substrate reaches its exhaustion state, thus reducing the signal. Compared to colorimetric detection, chemiluminescent detection is more sensitive.
A labeling enzyme, such as horseradish peroxidase, can be used to identify ABCB1 in a sample. Horseradish peroxidase (HRP), alkaline phosphatase, or catalase, have been used in ELISA for the ABCB1 marker. The choice of substrate is largely dependent on the sensitivity of the assay, and instrumentation used for signal detection.
LSBio ELISA kits are limited in shelf life. Unlike other ELISA kits, they are not stocked as finished goods. They are assemble upon ordering and tested prior to shipping. They may undergo minor changes to Range, Sensitivity, or Precision, which would be confirmed with the customer before shipping. Additionally, the lot numbers for the ELISA kits reflect the date of final assembly and testing.
An ELISA for the ABCB1 marker is a simple, reliable, and accurate way to measure the ABCB1 protein level in cancer cells. It is not a sandwich assay, but rather a multistep process. A patient's cells cultured overnight in a 96-well polystyrene plate is cultured for at least 24 hours to allow for a high-quality result.
This ELISA for the ABCB1 marker is a membrane-associated protein that belongs to the ABC transporter superfamily. This protein is a drug-transport pump that transports various molecules across membranes. It is located on chromosome 7 and has a mouse homolog, Mdr-3. These two markers have diverse functions, but they all work to help the body fight disease. The ABCB1 marker is a protein that can help in the treatment of different diseases.
PMID: 2876781 by Chen C.-J., et al. Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells.
PMID: 1967175 by Chen C.-J., et al. Genomic organization of the human multidrug resistance (MDR1) gene and origin of P-glycoproteins.
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